公开(公告)号：US20210301342A1

公开(公告)日：2021-09-30

申请号：US17268732

申请日：2019-09-06

Applicant: SEQUENOM, INC.

Inventor： Roy Brian Lefkowitz ,  John Allen Tynan ,  Chen Xu

IPC: C12Q1/6881 ,  G16B30/10 ,  G16B20/20 ,  G16H10/40 ,  G16H50/30 ,  G16H10/60 ,  G16H50/70

Abstract: This application provides methods and systems for determining transplant status. In some embodiments, the method comprises obtaining a biological sample from an organ transplant recipient who has received an organ; isolating cell-free nucleic acids from the biological sample; measuring the amount of each allele of one or more polymorphic nucleic acid targets in the biological sample; identifying the donor specific allele using a computer algorithm based on the measurements of the one or more polymorphic nucleic acid targets, whereby detecting one or more donor-specific circulating cell-free nucleic acids, detecting tissue injury based on the presence or amount of said one or more donor-specific nucleic acids, thereby determining transplant status.