公开(公告)号：US20150252365A1

公开(公告)日：2015-09-10

申请号：US14416559

申请日：2013-07-26

Applicant: SHANGHAI INSTITUTE OF MATERIA MEDICA, CHINESE ACADEMY OF SCIENCES

Inventor： Jin Ren ,  Yizheng Wang ,  Lingling Miao ,  Likun Gong ,  Xinming Qi ,  Chenggang Li ,  Guozhen Xing

IPC: C12N15/113

CPC classification number: C12N15/113 ,  C12N15/111 ,  C12N2310/141 ,  C12N2320/11

Abstract: Provided is a method for screening microRNAs with gene silencing function at both transcriptional and post-transcriptional levels. The microRNA obtained via the screening method can down-regulate the expression of mRNA of a target gene by binding to a specific site of 5′-flanking region of the target gene, and also down-regulate the expression of the protein of the target gene by binding to a specific site of the 3′-untranslated region of the target gene. Also provided are microRNAs obtained via the screening method, the use of the same in gene silencing, and a method of gene silencing by using the same.

Abstract translation: 提供了在转录和转录后水平上筛选具有基因沉默功能的microRNA的方法。 通过筛选方法获得的microRNA可以通过与目标基因的5'侧翼区域的特异位点结合而下调靶基因的mRNA表达，并且下调靶基因的蛋白质的表达 通过结合靶基因的3'非翻译区的特定位点。 还提供了通过筛选方法获得的微小RNA，其在基因沉默中的用途以及使用该基因沉默的方法。