公开(公告)号：US20240294929A1

公开(公告)日：2024-09-05

申请号：US18522708

申请日：2023-11-29

申请人： SHIHEZI UNIVERSITY

发明人： Genlin ZHANG ,  Yuanyuan LI ,  Jinyu ZHAO ,  Di QIU ,  Yan ZHANG

IPC分类号： C12N15/81 ,  C12P13/00 ,  C12P13/02

CPC分类号： C12N15/81 ,  C12P13/001 ,  C12P13/02

摘要： The present disclosure provides engineered Saccharomyces cerevisiae strains for production of vanillylamine and capsaicin, and a construction method and use thereof, and belongs to the technical field of bioengineering. In the present disclosure, a synthesis pathway of vanillylamine is expressed heterologously in Saccharomyces cerevisiae for the first time, and biosynthesis of the vanillylamine is realized for the first time. After the optimization of precursors supply and the optimization of S-adenosyl-L-methionine (SAM) cycle, a yield of the vanillylamine reaches 16.48 g/L, and a synthesis efficiency of the vanillylamine is increased by nearly 155%. This is the highest optimization efficiency obtained by optimizing the SAM cofactor strategy so far.