公开(公告)号：US20240353433A1

公开(公告)日：2024-10-24

申请号：US17928893

申请日：2021-12-30

申请人： SOOCHOW UNIVERSITY

发明人： Jianfeng GE ,  Mingsen ZHU ,  Qing YU ,  Ru SUN

IPC分类号： G01N33/92 ,  G01N33/533 ,  G01N33/58

CPC分类号： G01N33/92 ,  G01N33/533 ,  G01N33/582

摘要： The invention provides an amino-substituted chromenoquinoline-based fluorescent marker, and preparation and use thereof. The amino group of an aromatic amine compound is subjected to a one-step cyclization reaction with two o-chloroformylcumarins, to obtain a series of nitrogen-containing heterocyclic compound. Such compounds have excellent fluorescence emission performance, and also have good photostability in various organic solvents. The problem of photostability of cyanine dyes is effectively solved, and these dyes are useful in marking various organelles at cellular level. The introduction of the nitrogen-containing heterocyclic ring improves the biocompatibility of the compound, thus laying a good foundation for use in organisms. Further, the raw materials used in such reactions are cheap and readily available, the steps of synthesizing the marker are simple and easy to operate, and the production cost is effectively reduced while not lowering the performance of the marker. Therefore, the present invention is of commercial value.

公开(公告)号：US20230159819A1

公开(公告)日：2023-05-25

申请号：US17629599

申请日：2021-02-04

申请人： SOOCHOW UNIVERSITY

发明人： Jianfeng GE ,  Wei MA ,  Ru SUN

IPC分类号： C09K11/06 ,  G01N33/58

CPC分类号： C09K11/06 ,  G01N33/582 ,  C09K2211/1088 ,  C09K2211/1033

摘要： The present invention relates to a series of neutral fluorescent mitochondrial markers as amide derivatives, and a preparation method and use thereof. The present invention provides for the first time that neutral dyes have excellent mitochondria targeting ability after being bonded to an amide compound. The present invention solves the problems of uncertain organelle targeting ability of the existing fluorescent dyes with a neutral structure and that the neutral dye is a commercial marker for lipid droplets in cells. In the present invention, the organelle targeting ability of a fluorescent dye is regulated by creatively modifying the structure, while the optical performance of the original dye is improved; and the biological properties of the dye are obviously improved after being bonded to an amide compound. Amide compounds are cheap and readily available, which is conducive to controlling the cost of new dyes, thus having great scientific significance and commercial value.

公开(公告)号：US20220275274A1

公开(公告)日：2022-09-01

申请号：US17631432

申请日：2021-02-04

申请人： SOOCHOW UNIVERSITY

发明人： Jianfeng GE ,  Yue WANG ,  Ru SUN

IPC分类号： C09K11/06 ,  G01N21/64 ,  C07F5/02

摘要： The present invention provides a neutral fluorescent mitochondrial marker based on a nitrogen-containing heterocycle, and a preparation method and use thereof. The fluorophore in present invention is a heterocycle having a N—H bond and targeting mitochondria, which solves the problem that the ability of a fluorescent dye with a neutral structure to target organelles is random and uncertain, and also avoids the problem that the neutral fluorophore is a commercial marker for lipid droplets in cells. In the present invention, the organelle targeting ability of an original fluorophore is regulated by creatively modifying its structure while the optical performance of the fluorophore is improved. The marker improves the biological properties of the fluorophore, and the nitrogen-containing heterocycle building block is cheap and readily available, which is beneficial to controlling the cost of the new dye. The present invention has great scientific significance and commercial value.