Chemically hardened gelatin-separating medium for chromatography and ion exchange and method of making the same
    1.
    发明授权
    Chemically hardened gelatin-separating medium for chromatography and ion exchange and method of making the same 失效
    用于色谱和离子交换的化学硬化凝胶分离介质及其制备方法

    公开(公告)号:US3640809A

    公开(公告)日:1972-02-08

    申请号:US3640809D

    申请日:1968-12-10

    CPC classification number: B01J20/291 B01J20/22 B01J2220/54 G01N30/50

    Abstract: Selected gelatin, in particular the high molecular weight fraction obtained by fractionation with PEG is chemically hardened e.g. with tanning agents and used in a form of beads for exclusion chromatography and/or ion exchange or as a continuous body for electrophoresis. Ion exchange properties may be suppressed or enhanced as required by chemical treatment of the gelatin or by the choice of conditions during separation (pH, salt content). Ion-exchange properties are calibrated by electroosmosis.

    Abstract translation: 选择的明胶,特别是通过用PEG分级获得的高分子量级分被化学硬化,例如 使用鞣剂并以珠粒的形式用于排除色谱法和/或离子交换或用作电泳的连续体。 离子交换性能可通过明胶的化学处理或分离条件的选择(pH,盐含量)而被抑制或增强。 离子交换性质通过电渗校准。

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