公开(公告)号：US20210163958A1

公开(公告)日：2021-06-03

申请号：US16627760

申请日：2018-10-31

申请人： SOUTH CHINA UNIVERSITY OF TECHNOLOGY

发明人： Wenyong LOU ,  Xiaoyang OU ,  Minhua ZONG ,  Jiaxin GAO ,  Fei PENG ,  Pei XU

IPC分类号： C12N15/70 ,  C12N15/66 ,  C12Q1/686

摘要： The invention relates to a recombinant Escherichia coli expressing a fusion protein of formamidase and phosphite dehydrogenase, a construction method and use thereof. The invention includes adopting engineered E. coli DH5α as a host, amplifying a cloned formamidase gene and a cloned phosphite dehydrogenase gene into a fusion gene, ligating the fusion gene to a multiple cloning site of a vector, transforming the obtained recombinant plasmid into the E. coli DH5α, extracting the plasmid and transforming into an expression strain, and performing induction culture to obtain a recombinant E. coli. The recombinant E. coli can express a fusion protein of formamidase and phosphite dehydrogenase.

公开(公告)号：US20190040430A1

公开(公告)日：2019-02-07

申请号：US16083510

申请日：2016-12-15

申请人： SOUTH CHINA UNIVERSITY OF TECHNOLOGY

发明人： Ning LI ,  Yanmei LI ,  Minhua ZONG

IPC分类号： C12P17/04

摘要： A yeast strain and a method for the synthesis of 2,5-dihydroxymethylfuran using this strain are disclosed. The yeast strain is Meyerozyma guilliermondii SC 1103, which has been maintained in the China Center for Type Culture Collection (CCTCC, Wuhan, P.R. China) with an access No. of M2016144. The method for the synthesis of 2,5-dihydroxymethylfuran using this strain is described as follows: after pre-cultivation and cultivation, Meyerozyma guilliermondii SC 1103 cells are added into the buffer solutions containing 5-hydroxymethylfurfural and glucose; the biocatalytic reaction is conducted under designated conditions, thus affording 2,5-dihydroxymethylfuran. This disclosure has many advantages such as good selectivity, mild reaction conditions, environmental friendliness, high efficiency, and good yield.

公开(公告)号：US20220220518A1

公开(公告)日：2022-07-14

申请号：US17614331

申请日：2019-10-31

申请人： SOUTH CHINA UNIVERSITY OF TECHNOLOGY

发明人： Wenyong LOU ,  Linshang ZHANG ,  Minhua ZONG ,  Zifu NI ,  Jiguo YANG

IPC分类号： C12P7/64

摘要： Disclosed is a method for producing special oil OPO by way of microbial fermentation. The method comprises the following steps: inoculating microorganisms to an activating culture medium for shake cultivation treatment to obtain an activated culture; adding an organic matter into a minimal medium to obtain an optimized culture medium; inoculating the culture into the optimized culture medium for shake cultivation treatment to obtain a culture solution; carrying out centrifugal treatment on the culture solution, taking a precipitate, washing the precipitate and freeze-drying the precipitate, adding a hydrochloric acid solution to obtain a mixture; and adding an organic solvent into the mixture for extraction, and removing the solvent to obtain the OPO, wherein the microorganisms are Rhodococcus opacus PD630.