公开(公告)号：US20030022263A1

公开(公告)日：2003-01-30

申请号：US10024123

申请日：2001-12-17

Applicant: ST. JUDE CHILDREN'S RESEARCH HOSPITAL

Inventor： Michael Kastan ,  Christine Canman ,  Seong-Tae Kim ,  Dae-Sik Lim

IPC: C12Q001/48

CPC classification number: C12Q1/48 ,  G01N33/573 ,  G01N2333/9121

Abstract: The present invention relates to identification of the consensus sequence phosphorylated by ATM kinase. This, in turn, permitted identification of ATM kinase target proteins, and development of a convenient assay system for ATM kinase phosphorylation using fusion polypeptides as substrates. The assay system is adaptable to screening for ATM modulators, particularly inhibitors. In a specific embodiment, the substrate recognition sequence and mutagenized variants of this sequence were incorporated in a GST fusion protein and assayed for phosphorylation by ATM kinase. This assay system is useful in screening for ATM inhibitors. ATM function assays were validated using an ATM-kinase dead dominant-negative mutant.