公开(公告)号：US3642582A

公开(公告)日：1972-02-15

申请号：US3642582D

申请日：1969-07-14

Applicant: STANDARD BRANDS INC

Inventor： MCCLARY JOSEPH E

IPC: C12N9/28 ,  C12N9/56 ,  C07G7/02

CPC classification number: C12N9/2417 ,  C12N9/54 ,  Y10S435/815 ,  Y10S435/839

Abstract: Aqueous alpha-amylase preparations may be purified by removing the polyvalent anions therefrom, and contacting the preparations with a fibrous alumina of the kind described in U.S. Pat. No. 2,915,475. The alpha-amylase will be adsorbed on the alumina and may subsequently be recovered therefrom by washing the same with a salt solution containing polyvalent anions. In the case where the alpha-amylase preparation is derived from fermentation of B. subtilis, protease is present in large quantities and when such a preparation is contacted with fibrous alumina the protease will remain in the effluent and can be recovered therefrom by wellknown methods.

Abstract translation: 水性α-淀粉酶制剂可以通过从其中除去多价阴离子来纯化，并使制剂与美国专利中描述的纤维状氧化铝接触。 第2,915,475号。 α-淀粉酶将被吸附在氧化铝上，随后可以通过用含有多价阴离子的盐溶液洗涤它们来回收。 在α-淀粉酶制剂衍生自枯草芽孢杆菌的发酵的情况下，蛋白酶大量存在，并且当这种制剂与纤维状氧化铝接触时，蛋白酶将保留在流出物中并且可以通过公知的方法从其中回收 方法。