公开(公告)号：US20180110831A1

公开(公告)日：2018-04-26

申请号：US15557000

申请日：2016-03-09

Applicant: STC.UNM

Inventor： C. Jeffrey Brinker ,  Eric C. Carnes ,  Carlee Erin Ashley ,  Jacob Ongundi Agola ,  Kimberly Butler ,  Christophe Theron

IPC: A61K38/17 ,  A61K47/64 ,  A61K47/69 ,  A61K9/51 ,  C07K14/705

CPC classification number: A61K38/1774 ,  A61K9/127 ,  A61K9/5115 ,  A61K9/5123 ,  A61K47/6425 ,  A61K47/6911 ,  B82Y5/00 ,  C07K14/70546

Abstract: The present invention is directed to protocells, which have a core and a lipid bilayer surrounding the core, with at least one CD47 molecule or an active fragment thereof in or conjugated to the lipid bilayer. The CD47 present on the lipid bilayer allows the protocell to evade phagocytosis by macrophages, and can be conjugated to the lipid bilayer via a crosslinker. The protocell can be loaded with a diagnostic or therapeutic cargo, such as a polypeptide, a nucleic acid, or a drug. The protocell can also include a targeting species for targeted delivery of the cargo to a cell. The protocell can also include an endosomolytic peptide, which promotes endosomal escape after uptake by the targeted cell. The protocells with CD47 on the lipid bilayer provide better circulation after in vivo administration compared to protocells without CD47, and are therefore particularly useful as a cargo delivery vehicle.