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    Expression method of Haemocoagulase Acutus (Halase) recombinant protein
    5.
    发明授权
    Expression method of Haemocoagulase Acutus (Halase) recombinant protein 有权

    公开(公告)号：US11499163B2

    公开(公告)日：2022-11-15

    申请号：US16175812

    申请日：2018-10-30

    Applicant: SUN YAT-SEN UNIVERSITY

    Inventor： Qin Deng ,  Xiao Shen ,  Weiwei Su ,  Zhong Wu ,  Wei Peng ,  Yonggang Wang

    IPC: C12N15/85 ,  C12N15/64 ,  G01N33/573 ,  C12N9/64

    Abstract: The disclosure relates to an expression method of a Haemocoagulase Acutus (Halase) recombinant protein. The method includes the following steps: (1) optimizing a halase gene; (2) performing Polymerase Chain Reaction (PCR) amplification on an optimized halase gene; (3) constructing an Agkis-pMCX expression vector, transforming plasmids to competent cells of an Escherichia coli for amplification, screening in an Amp-resistant manner for positive cloning, sequencing and extracting recombinant plasmids with correct sequencing; (4) transfecting recombinant plasmids to CHO cells; and (5) expressing the recombinant protein and identifying. According to the expression method, a recombinant halase is expressed first using the CHO cells cultured in a serum-free suspension manner; and by utilizing a bioengineering means, the actual production problems of insufficient raw material sources and unstable quality of a snake venom product are solved successfully.

    EXPRESSION METHOD OF HAEMOCOAGULASE ACUTUS (HALASE) RECOMBINANT PROTEIN
    9.
    发明申请
    EXPRESSION METHOD OF HAEMOCOAGULASE ACUTUS (HALASE) RECOMBINANT PROTEIN 审中-公开

    公开(公告)号：US20190048361A1

    公开(公告)日：2019-02-14

    申请号：US16175812

    申请日：2018-10-30

    Applicant: SUN YAT-SEN UNIVERSITY

    Inventor： Qin Deng ,  Xiao Shen ,  Weiwei Su ,  Zhong Wu ,  Wei Peng ,  Yonggang Wang

    IPC: C12N15/85 ,  G01N33/573 ,  C12N15/64

    Abstract: The disclosure relates to an expression method of a Haemocoagulase Acutus (Halase) recombinant protein. The method includes the following steps: (1) optimizing a halase gene; (2) performing Polymerase Chain Reaction (PCR) amplification on an optimized halase gene; (3) constructing an Agkis-pMCX expression vector, transforming plasmids to competent cells of an escherichia coli for amplification, screening in an Amp-resistant manner for positive cloning, sequencing and extracting recombinant plasmids with correct sequencing; (4) transfecting recombinant plasmids to CHO cells; and (5) expressing the recombinant protein and identifying. According to the expression method, a recombinant halase is expressed first using the CHO cells cultured in a serum-free suspension manner; and by utilizing a bioengineering means, the actual production problems of insufficient raw material sources and unstable quality of a snake venom product are solved successfully.

    Method for preparing solution formulation for aerosol inhalation of naringenin
    10.
    发明授权
    Method for preparing solution formulation for aerosol inhalation of naringenin 有权

    公开(公告)号：US12064412B2

    公开(公告)日：2024-08-20

    申请号：US16976728

    申请日：2019-08-28

    Applicant: SUN YAT-SEN UNIVERSITY

    Inventor： Mingyi Guan ,  Weiwei Su ,  Hongliang Yao

    IPC: A61K31/352 ,  A61K9/00 ,  A61K9/08 ,  A61K47/02 ,  A61K47/12 ,  A61K47/18 ,  A61K47/40

    CPC classification number: A61K31/352 ,  A61K9/0078 ,  A61K9/08 ,  A61K47/02 ,  A61K47/12 ,  A61K47/186 ,  A61K47/40

    Abstract: The present invention discloses a solution formulation for aerosol inhalation of naringenin and preparation method thereof. The formulation is prepared from 1 part by weight of naringenin, 15-30 parts by weight of hydroxypropyl β-cyclodextrin, a buffer-salt solution and an appropriate amount of an excipient. The preparation method includes: preparing a buffer-salt solution of a pH value of 7-8.5 by using the buffer salt, adding the naringenin into the buffer-salt solution, then adding the hydroxypropyl β-cyclodextrin, shaking in a constant-temperature air bath till complete dissolving and coating, adding an appropriate amount of the excipient, filtering, filling and sterilizing. The present invention, by firstly increasing the solubility of the free naringenin in the solvent by adjusting the pH value, and then coating the naringenin with the hydroxypropyl β-cyclodextrin, significantly increases the overall concentration of the naringenin in the solvent.

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