公开(公告)号：US20200045977A1

公开(公告)日：2020-02-13

申请号：US16339866

申请日：2017-09-28

申请人： SYNGENTA PARTICIPATIONS AG ,  Devgen NV

发明人： Pascale FELDMANN ,  Jeffrey David FOWLER ,  Nema Devi JHURRY ,  Isabelle MAILLET ,  Marta OMEDES PUJOL

IPC分类号： A01N63/02 ,  C12N15/10 ,  C12N15/11

摘要： The present invention provides a method of substantially retaining or otherwise preserving the biological activity of a dsRNA, present in a cell, to post-transcriptionally silence the expression of a gene in a target organism, comprising the step of adding to the cell composition a compound having the function of a protein—or amine—cross linking agent and/or an acid. The invention also comprises compositions comprising the cells comprising dsRNA, and protein cross linking agents and/or acids, as well as the use of said cross linking agents and/or acids in the method.

公开(公告)号：US20200040335A1

公开(公告)日：2020-02-06

申请号：US16339989

申请日：2017-09-19

申请人： SYNGENTA PARTICIPATIONS AG

发明人： Jeffrey David FOWLER ,  Nema Devi JHURRY

IPC分类号： C12N15/113 ,  A01N63/02 ,  A01N25/22

摘要： The present invention provides for methods of delivering polynucleotides to subterranean plant pests. In particular, the present invention relates to methods of reducing the binding of a composition, comprising polynucleotide and a cationic polymer, to soil by substantially quenching positively charged residues with a quenching agent. The invention also comprises compositions comprising a cationic polymer, a polynucleotide and a quenching agent.