公开(公告)号：US5818582A

公开(公告)日：1998-10-06

申请号：US818881

申请日：1997-03-17

申请人： Salvador M. Fernandez ,  Ernest St. Louis ,  Ralph Levy ,  Ernest F. Gulgon ,  Sean Cobane

发明人： Salvador M. Fernandez ,  Ernest St. Louis ,  Ralph Levy ,  Ernest F. Gulgon ,  Sean Cobane

IPC分类号： G01J3/44 ,  G01J3/30

CPC分类号： G01J3/4406

摘要： A phase fluorometer employs a baseband frequency generator to generate a sample baseband signal at a first frequency f.sub.o and a reference signal correlated to the sample baseband signal. A second signal generator generates a carrier signal having a second frequency f. The sample baseband signal and the carrier signal are combined to form an up-converted sample signal having a frequency f.+-.f.sub.o. A light source is modulated at the up-converted sample signal frequency f.+-.f.sub.o to illuminate a sample. A photo detector detects fluorescence emissions from a sample excited by the excitation light and converts the emission into a fluorescence signal. A down-converter combines the carrier signal and fluorescence signal to form a phase-shifted and down-converted sample signal. A processor digitally processes the phase-shifted and demodulated sample signal and the reference signal to determine the fluorescence lifetime.

摘要翻译： 相位荧光计采用基带频率发生器产生第一频率fo的采样基带信号和与采样基带信号相关的参考信号。 第二信号发生器产生具有第二频率f的载波信号。 样本基带信号和载波信号被组合以形成具有频率f +/- fo的上变频采样信号。 在上转换的采样信号频率f +/- fo处调制光源以照射样品。 光检测器检测由激发光激发的样品的荧光发射，并将发射转换成荧光信号。 下转换器组合载波信号和荧光信号以形成相移和下变换的采样信号。 处理器对相移和解调的采样信号和参考信号进行数字处理，以确定荧光寿命。