公开(公告)号：US20220248663A1

公开(公告)日：2022-08-11

申请号：US17626381

申请日：2020-07-13

申请人： Sanaria Inc.

发明人： Eric R. JAMES

IPC分类号： A01N1/02 ,  A01K67/033

摘要： Herein is described a method devised to cryopreserve mosquito eggs, viable upon subsequent thawing. The method reproducibly yields >25% hatched first instar larvae of Anopheles-species mosquitoes from cryopreserved eggs. The majority of hatched larvae continue to develop normally (87% pupation), through to adults (98%, 50% of which are female) which subsequently blood feed and produce viable second generation embryos that also develop normally. Adult mosquitoes obtained from cryopreserved embryos are able to be infected with Plasmodium falciparum gametocytes and produce salivary gland sporozoites in similar numbers to control mosquitoes.