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    Alpha-1-antitrypsin compositions
    1.
    发明申请
    Alpha-1-antitrypsin compositions 有权
    α-1-抗胰蛋白酶组合物

    公开(公告)号：US20120165261A1

    公开(公告)日：2012-06-28

    申请号：US13385642

    申请日：2012-02-28

    申请人： Scott M. Kee ,  Paul I. Cook ,  James R. Smith ,  Robert Kling ,  Scott A. Fowler ,  David Weber

    发明人： Scott M. Kee ,  Paul I. Cook ,  James R. Smith ,  Robert Kling ,  Scott A. Fowler ,  David Weber

    IPC分类号： A61K38/17 ,  A61P11/00

    CPC分类号： A61K38/57 ,  A61K9/19 ,  B01D15/327 ,  B01D15/363 ,  C07K14/8125 ,  G01N30/461

    摘要： A streamlined method for purifying alpha-1-antitrypsin (AAT) from an AAT-containing protein mixture, such as a Cohn fraction IV precipitate, is provided. In the method of the invention, contaminating proteins are destabilized by cleavage of disulfide bonds with a reducing reagent, such as a dithiol, which does not affect AAT. The destabilized proteins are then preferentially adsorbed on a solid protein-adsorbing material, without the addition of a salt as a precipitant. Separation of the solid adsorbent from the solution leaves a purified AAT solution that is directly suitable for chromatographic purification, without the need for extensive desalting as in prior art processes. A process incorporating this method, which provides pharmaceutical grade AAT in high yield on a commercial scale, is also described.

    摘要翻译： 提供了从含有AAT的蛋白质混合物如Cohn馏分IV沉淀物中纯化α-1-抗胰蛋白酶（AAT）的流线型方法。 在本发明的方法中，污染蛋白质通过用不影响AAT的还原剂如二硫醇裂解二硫键而不稳定。 然后将不稳定的蛋白质优先吸附在固体蛋白质吸附材料上，而不加入作为沉淀剂的盐。 固体吸附剂与溶液的分离留下直接适用于色谱纯化的纯化AAT溶液，而不需像现有技术方法那样进行广泛的脱盐。 还描述了一种以商业规模提供高产量的药物级AAT的方法。

    Method for purification of alpha-1-antitrypsin
    2.
    发明授权
    Method for purification of alpha-1-antitrypsin 有权
    纯化α-1-抗胰蛋白酶的方法

    公开(公告)号：US07777006B2

    公开(公告)日：2010-08-17

    申请号：US10334303

    申请日：2002-12-31

    申请人： Scott M. Kee ,  Paul I. Cook ,  James R. Smith ,  Robert Kling ,  Scott A. Fowler ,  David Weber

    发明人： Scott M. Kee ,  Paul I. Cook ,  James R. Smith ,  Robert Kling ,  Scott A. Fowler ,  David Weber

    IPC分类号： A61K35/14

    CPC分类号： A61K38/57 ,  A61K9/19 ,  B01D15/327 ,  B01D15/363 ,  C07K14/8125 ,  G01N30/461

    摘要： A streamlined method for purifying alpha-1-antitrypsin (AAT) from an AAT-containing protein mixture, such as a Cohn fraction IV precipitate, is provided. In the method of the invention, contaminating proteins are destabilized by cleavage of disulfide bonds with a reducing reagent, such as a dithiol, which does not affect AAT. The destabilized proteins are then preferentially adsorbed on a solid protein-adsorbing material, without the addition of a salt as a precipitant. Separation of the solid adsorbent from the solution leaves a purified AAT solution that is directly suitable for chromatographic purification, without the need for extensive desalting as in prior art processes. A process incorporating this method, which provides pharmaceutical grade AAT in high yield on a commercial scale, is also described.

    摘要翻译： 提供了从含有AAT的蛋白质混合物如Cohn馏分IV沉淀物中纯化α-1-抗胰蛋白酶（AAT）的流线型方法。 在本发明的方法中，污染蛋白质通过用不影响AAT的还原剂如二硫醇裂解二硫键而不稳定。 然后将不稳定的蛋白质优先吸附在固体蛋白质吸附材料上，而不加入作为沉淀剂的盐。 固体吸附剂与溶液的分离留下直接适用于色谱纯化的纯化AAT溶液，而不需像现有技术方法那样进行广泛的脱盐。 还描述了一种以商业规模提供高产量的药物级AAT的方法。