公开(公告)号：US20210054368A1

公开(公告)日：2021-02-25

申请号：US16856947

申请日：2020-04-23

Applicant: Seagate Technology LLC

Inventor： Tim RAUSCH ,  Walter R EPPLER ,  Gemma MENDONSA

IPC: C12N15/10 ,  G16B50/30 ,  G06F12/02

Abstract: A system for DNA gene assembly that utilizes a DNA symbol library and a DNA linker library. The symbol library has a number of DNA symbols each having a first overhanging end and a second overhanging end different than and non-complimentary to the first end, the first and second ends being the same nucleotides for each DNA symbol. The linker library has pairs of DNA linkers, a first linker of a pair having a first overhanging end and a second overhanging end and a second linker of the pair having a first overhanging end and a second overhanging end, the first end of the first linker being the same nucleotides for each first linker and the second end of the second linker being the same nucleotides for each second linker, wherein the second end of the first linker and the first end of the second linker have complementary nucleotides. The first linker joins to the first end of a DNA symbol and the second linker joins to the second end of another DNA symbol.

公开(公告)号：US20210309991A1

公开(公告)日：2021-10-07

申请号：US17348642

申请日：2021-06-15

Applicant: Seagate Technology LLC

Inventor： Tim RAUSCH ,  Walter R EPPLER ,  Gemma MENDONSA

IPC: C12N15/10 ,  C40B60/14 ,  B01L3/00 ,  G16B50/30 ,  G06F12/02 ,  G11C13/02

Abstract: A system for DNA gene assembly that utilizes a DNA symbol library and a DNA linker library. The symbol library has a number of DNA symbols each having a first overhanging end and a second overhanging end different than and non-complimentary to the first end, the first and second ends being the same nucleotides for each DNA symbol. The linker library has pairs of DNA linkers, a first linker of a pair having a first end and a second end and a second linker of the pair having a first end and a second end, the first end of the first linker being the same nucleotides for each first linker and the second end of the second linker being the same nucleotides for each second linker, wherein the second end of the first linker and the first end of the second linker have complementary nucleotides. The first linker joins to the first end of a DNA symbol and the second linker joins to the second end of another DNA symbol.

公开(公告)号：US20210054364A1

公开(公告)日：2021-02-25

申请号：US16857006

申请日：2020-04-23

Applicant: Seagate Technology LLC

Inventor： Tim RAUSCH ,  Walter R EPPLER ,  Gemma MENDONSA

IPC: C12N15/10 ,  B01L3/00 ,  C40B60/14

Abstract: A microfluidic lab-on-a-chip system for DNA gene assembly that utilizes a DNA symbol library and a DNA linker library. The lab-on-a-chip has a fluidic platform with a plurality of arrays operably connected to a voltage source and a controller for the voltage source, a set of first inlets operably connected to the fluidic platform, each first inlet for one DNA symbol from a DNA symbol library, a set of second inlets operably connected to the fluidic platform, each second inlet for one DNA linker from a DNA linker library, and a mixing area operably connected to the fluidic platform and to the plurality of first inlets and the plurality of second inlets.