公开(公告)号：US20230028798A1

公开(公告)日：2023-01-26

申请号：US17787948

申请日：2020-12-21

申请人： Shanghai Ninth People's Hospital, Shanghai JiaoTong University School of Medicine

发明人： Qingfeng LI ,  Shuangbai ZHOU

IPC分类号： A01N1/02 ,  C12N5/077

摘要： Use of glycerol in preparing an adipose tissue cryopreservation protective agent and the adipose tissue cryopreservation protective agent. Based on the total volume of the adipose tissue cryopreservation protective agent, the adipose tissue cryopreservation protective agent includes: glycerol with a volume fraction of 60-80%; 0.090-0.200 g/ml trehalose; and a PBS buffer serving as the solvent. The adipose tissue cryopreservation protective agent is free of DMSO and other protective agents having biological toxicity, poses no toxicity to cells and tissues, and causes no safety problem during clinical use even in a case of incomplete elution. The adipose tissue cryopreservation protective agent is free of xenoantigen and has low cost. With glycerol and trehalose being clinically approved medicinal ingredients and inexpensive products, the agent of the present disclosure contains no costly products such as human albumin, thus providing increased possibility for clinical use and promotion.