公开(公告)号：US20100269224A1

公开(公告)日：2010-10-21

申请号：US12763790

申请日：2010-04-20

申请人： Shihshieh Huang ,  Stanislaw Flasinski ,  Alessandra Frizzi ,  Brad Gabor ,  Charles Hagen ,  John Kao ,  Raquel Salati

发明人： Shihshieh Huang ,  Stanislaw Flasinski ,  Alessandra Frizzi ,  Brad Gabor ,  Charles Hagen ,  John Kao ,  Raquel Salati

IPC分类号： A01H5/00 ,  C12N15/82 ,  C12N5/10

CPC分类号： C12N15/8283 ,  C12N15/8218

摘要： The present invention provides gene targets, constructs and methods for the genetic control of plant disease caused by multiple plant viruses. The present invention relates to achieving a plant protective effect through the identification of target coding sequences and the use of recombinant DNA technologies for post-transcriptionally repressing or inhibiting expression of the target coding sequences of plant-parasitic viruses. Protein-expression based approaches may also be utilized to augment phenotype resistance. Thus, transcription of a single transgenic event comprising one or more plant expression cassettes can allow for broad spectrum resistance of a plant to multiple plant viral strains and species among the geminiviruses, tospoviruses, and potexviruses.

摘要翻译： 本发明提供了由多种植物病毒引起的植物病害遗传控制的基因靶标，构建体​​和方法。 本发明涉及通过鉴定靶编码序列和重组DNA技术用于转录后抑制或抑制植物寄生病毒靶标编码序列的表达来实现植物保护作用。 也可以利用基于蛋白质表达的方法来增加表型抗性。 因此，包含一个或多个植物表达盒的单个转基因事件的转录可以允许植物对多种植物病毒株和双系疱疹病毒，鸟毒病毒和盆病毒中的物种的广谱抗性。

公开(公告)号：US08455716B2

公开(公告)日：2013-06-04

申请号：US12763790

申请日：2010-04-20

申请人： Shihshieh Huang ,  Stanislaw Flasinski ,  Alessandra Frizzi ,  Brad Gabor ,  Charles Hagen ,  John Kao ,  Raquel Salati

发明人： Shihshieh Huang ,  Stanislaw Flasinski ,  Alessandra Frizzi ,  Brad Gabor ,  Charles Hagen ,  John Kao ,  Raquel Salati

IPC分类号： C12N15/82 ,  C12N15/113 ,  C12N5/10 ,  A01H5/00

CPC分类号： C12N15/8283 ,  C12N15/8218

摘要： The present invention provides gene targets, constructs and methods for the genetic control of plant disease caused by multiple plant viruses. The present invention relates to achieving a plant protective effect through the identification of target coding sequences and the use of recombinant DNA technologies for post-transcriptionally repressing or inhibiting expression of the target coding sequences of plant-parasitic viruses. Protein-expression based approaches may also be utilized to augment phenotype resistance. Thus, transcription of a single transgenic event comprising one or more plant expression cassettes can allow for broad spectrum resistance of a plant to multiple plant viral strains and species among the geminiviruses, tospoviruses, and potexviruses.

摘要翻译： 本发明提供了由多种植物病毒引起的植物病害遗传控制的基因靶标，构建体​​和方法。 本发明涉及通过鉴定靶编码序列和重组DNA技术用于转录后抑制或抑制植物寄生病毒靶标编码序列的表达来实现植物保护作用。 也可以利用基于蛋白质表达的方法来增加表型抗性。 因此，包含一个或多个植物表达盒的单个转基因事件的转录可以允许植物对多种植物病毒株和双系疱疹病毒，鸟毒病毒和盆病毒中的物种的广谱抗性。