公开(公告)号：US5091309A

公开(公告)日：1992-02-25

申请号：US395007

申请日：1989-08-17

申请人： Sondra Schlesinger ,  Henry V. Huang ,  Robin Levis ,  Barbara Weiss ,  Manuel Tsiang

发明人： Sondra Schlesinger ,  Henry V. Huang ,  Robin Levis ,  Barbara Weiss ,  Manuel Tsiang

IPC分类号： C07K14/18 ,  C12N15/86

CPC分类号： C07K14/005 ,  C12N15/86 ,  C12N2770/36122 ,  C12N2770/36143 ,  Y10S435/948

摘要： The present invention relates to RNA vectors comprising Sindbis virus DI RNA having inserted therein heterologous RNA. The vectors of the present invention provide for the cloning and expression of heterologous RNA in eucaryotic cells as well as the packaging of heterologous RNA into viral particles in the presence of helper virus. Transformed cells and genetically altered alphavirus particles comprising alphavirus DI RNA having inserted therein heterologous RNA are also provided.

摘要翻译： 本发明涉及包含插入有异源RNA的辛德比斯病毒DI RNA的RNA载体。 本发明的载体提供了在真核细胞中克隆和表达异源RNA以及在辅助病毒存在下将异源RNA包装到病毒颗粒中。 还提供了转化的细胞和遗传改变的甲病毒颗粒，其包含插入其中的异源RNA的甲病毒属RNA。

公开(公告)号：US5217879A

公开(公告)日：1993-06-08

申请号：US815602

申请日：1991-12-27

申请人： Henry V. Huang ,  Robin Levis ,  Charles M. Rice ,  Sondra Schlesinger ,  Ping Shen ,  Cheng Xiong

发明人： Henry V. Huang ,  Robin Levis ,  Charles M. Rice ,  Sondra Schlesinger ,  Ping Shen ,  Cheng Xiong

IPC分类号： C12N7/01 ,  C12N15/86

CPC分类号： C12N15/86 ,  C12N2760/18843

摘要： Compositions and methods are disclosed for expressing heterologous coding sequences in host cells. The compositions include infectious Sindbis virus RNA molecules containing at least one heterologous coding sequence inserted within the structural region of the Sindbis virus genome. RNA molecules consisting essentially of a Sindbis virus junction region are also provided. Methods utilizing the novel compositions of the present invention to express heterologous coding sequences in transformed host cells are provided. Methods for producing infectious Sindbis virus particles containing infectious Sindbis virus RNA molecules comprising at least one heterologous coding sequence are provided.This invention was made with government support under grant numbers AI24134, AI11377 and AG05681 awarded by the National Institutes of Health. The government has certain rights in the invention.

摘要翻译： 公开了用于在宿主细胞中表达异源编码序列的组合物和方法。 组合物包括感染性的辛德比斯病毒RNA分子，其含有插入在辛德比斯病毒基因组的结构区域内的至少一个异源编码序列。 还提供了基本上由辛德毕斯病毒连接区组成的RNA分子。 提供了利用本发明新组合物在转化的宿主细胞中表达异源编码序列的方法。 提供了包含至少一种异源编码序列的含感染性辛德毕斯病毒RNA分子的感染性辛德比斯病毒颗粒的方法。