公开(公告)号：US20190284576A1

公开(公告)日：2019-09-19

申请号：US16319216

申请日：2017-07-21

Applicant: Spark Therapeutics, Inc.

Inventor： Guang QU ,  John Fraser WRIGHT ,  Younghoon OH ,  Yuhuan WANG ,  Haibo ZHANG ,  Laura DUNCAN

IPC: C12N15/86 ,  C07K14/755 ,  C12N9/64 ,  C12N9/48

Abstract: Provided are methods for producing recombinant adeno-associated virus (rAAV) vector particles at high recovery or high titer. Also provided are methods that concentrate rAAV vectors to a high concentration, for example, up to 5E+13 (5×1013) vector genomes per milliliter (Vg/ml) with little if any rAAV aggregates.