公开(公告)号：US20250145951A1

公开(公告)日：2025-05-08

申请号：US18837739

申请日：2023-02-16

Applicant: StemCell Technologies Canada Inc.

Inventor： Hitesh ARORA ,  Mark BROWN

IPC: C12N5/0783 ,  C12N5/0781

Abstract: Disclosed are media, supplements and methods for expanding mammalian lymphocytes or progenitors thereof, such as B cells, T cells, or NK cells. The disclosed media, supplements and methods may be used to expand mammalian lymphocytes or progenitors thereof plated as single cells, or at a clonal cell density. In some embodiments, the disclosed media, supplements and methods may also differentiate/activate the lymphocytes during expansion. The media, supplements and methods of this disclosure may be used in serum-free and feeder-free culture workflows.