公开(公告)号：US20100173793A1

公开(公告)日：2010-07-08

申请号：US12063803

申请日：2006-08-17

申请人： Suzanne Jane Dilly ,  Paul Christopher Taylor ,  Andrew James Clark ,  Andrew Marsh ,  Richard Napier ,  Andrew Thompson

发明人： Suzanne Jane Dilly ,  Paul Christopher Taylor ,  Andrew James Clark ,  Andrew Marsh ,  Richard Napier ,  Andrew Thompson

IPC分类号： C40B30/04 ,  C07D229/02 ,  C08G63/91 ,  C08G69/48 ,  B01L3/00 ,  C40B40/10

CPC分类号： G01N33/6845 ,  G01N33/54353 ,  G01N2500/04

摘要： The invention provides a method of identifying a peptide or protein capable of binding a ligand which comprises: (i) providing a support, the support comprising a photoreactive group; (ii) reacting the photoreactive group with a ligand to attach the ligand to the support and produce a supported ligand; (iii) providing an expression library comprising a plurality of members, each member expressing a different peptide or protein; (iv) screening the expression library to identify one or more peptides or proteins which bind to the ligand; (v) isolating the member or each member of the library which expresses a peptide or protein which binds to the ligand; and (vi) identifying the peptide or protein which binds to the ligand. Supported photo-reactive compounds are disclosed comprising a photo-reactive group attached to a support via a spacer and a dendritic group, the dendritic group comprising attached thereto, optionally via a spacer, at least one further photo-reactive group and/or a second functional group. Compounds comprising photo-reactive groups attached to a support and a protein resistant group attached to the support are also provided, together with kits for carrying out the method of the invention.

摘要翻译： 本发明提供了鉴定能够结合配体的肽或蛋白质的方法，其包括：（i）提供载体，所述载体包含光反应性基团; （ii）使光反应性基团与配体反应以将配体连接到载体上并产生负载的配体; （iii）提供包含多个成员的表达文库，每个成员表达不同的肽或蛋白质; （iv）筛选表达文库以鉴定结合配体的一种或多种肽或蛋白质; （v）分离表达与配体结合的肽或蛋白质的文库的成员或每个成员; 和（vi）鉴定与配体结合的肽或蛋白质。 公开了支持的光反应性化合物，其包括通过间隔基和树枝状基团连接到载体上的光反应性基团，所述树枝状基团任选地经由间隔基，至少一个另外的光反应性基团和/或第二 功能组。 还提供了包含连接到载体上的光反应性基团和与载体连接的抗蛋白质基团的化合物以及用于实施本发明方法的试剂盒。

公开(公告)号：US09987964B1

公开(公告)日：2018-06-05

申请号：US15728626

申请日：2017-10-10

申请人： Richard Napier

发明人： Richard Napier

IPC分类号： B60P3/10 ,  B62D63/08 ,  B60S9/04 ,  B60Q1/00 ,  B62D63/06

CPC分类号： B60P3/1033 ,  B60Q1/0088 ,  B60S9/04 ,  B62D63/062 ,  B62D63/08

摘要： The boat trailer having an inflatable bladder is a boat trailer with an inflatable bladder located under the rear end of the trailer. The bladder is coupled to an air hose that runs to a hose connecter at the front of the trailer. The bladder is retained in place by a combination of longitudinal and lateral support rails, which are part of the trailer frame and by a bladder barrier that covers the bladder and prevents it from rising above the top of the trailer. If the trailer is backed down the boat ramp and the wheels leave the boat ramp at an end-of-ramp drop-off, a source of compressed air may be used to inflate the bladder. This provides buoyancy to elevate the trailer wheels so that the tow vehicle may pull the trailer up the boat ramp.