公开(公告)号：USRE32271E

公开(公告)日：1986-10-28

申请号：US727807

申请日：1985-04-26

申请人： Syed S. Husain ,  Boguslaw Lipinski ,  Victor Gurewich

发明人： Syed S. Husain ,  Boguslaw Lipinski ,  Victor Gurewich

IPC分类号： A61K38/00 ,  C12N9/72

CPC分类号： C12N9/6456 ,  A61K38/00

摘要： The existence of high fibrin-affinity urokinase is discovered by an isolation procedure using fibrin precipitated on an adsorptive-solid matrix. By the method described, the high affinity form of plasminogen activator can be isolated directly from urine or from kidney tissue culture medium. The method is economical and provides a relatively high yield of the activator. The high affinity that this plasminogen activator has for fibrin is a property that makes it an improved thrombolytic agent and when radiolabelled provides a new diagnostic agent for the specific detection of fibrin thrombi through nuclear scanning. The newly-isolated plasminogen activator has the following characteristics: a molecular weight of about 56,000 Daltons, a specific activity of about 40,000-50,000 CTA units/mg, the appearance of a single chain structure and a high affinity for fibrin.

公开(公告)号：US4381346A

公开(公告)日：1983-04-26

申请号：US182976

申请日：1980-09-02

申请人： Syed S. Huasin ,  Boguslaw Lipinski ,  Victor Gurewich

发明人： Syed S. Huasin ,  Boguslaw Lipinski ,  Victor Gurewich

IPC分类号： A61K38/00 ,  C12N9/72 ,  C12N9/48

CPC分类号： C12N9/6456 ,  A61K38/00

摘要： The existence of high fibrin-affinity urokinase is discovered by an isolation procedure using fibrin precipitated on an adsorptive-solid matrix. By the method described, the high affinity form of plasminogen activator can be isolated directly from urine or from kidney tissue culture medium. The method is economical and provides a relatively high yield of the activator. The high affinity that this plasminogen activator has for fibrin is a property that makes it an improved thrombolytic agent and when radiolabelled provides a new diagnostic agent for the specific detection of fibrin thrombi through nuclear scanning. The newly-isolated plasminogen activator has the following characteristics: a molecular weight of about 56,000 Daltons, a specific activity of about 40,000-50,000 CTA units/mg, the appearance of a single chain structure and a high affinity for fibrin.

摘要翻译： 高纤维蛋白亲和性尿激酶的存在是通过使用沉淀在吸附固体基质上的纤维蛋白的分离方法发现的。 通过所述方法，纤溶酶原激活物的高亲和力形式可直接从尿或肾组织培养基中分离。 该方法是经济的并且提供了相对高的活化剂产率。 这种纤溶酶原激活剂对纤维蛋白的高亲和力是使其成为改进的溶栓剂的特性，当放射性标记为核扫描的纤维蛋白血栓特异性检测提供了新的诊断剂。 新分离的纤溶酶原激活物具有以下特征：约56,000道尔顿的分子量，约40,000-50,000 CTA单位/ mg的比活性，单链结构的外观和对纤维蛋白的高亲和力。

公开(公告)号：US5607841A

公开(公告)日：1997-03-04

申请号：US262607

申请日：1994-06-20

申请人： Boguslaw Lipinski

发明人： Boguslaw Lipinski

IPC分类号： C07K2/00 ,  C07K14/47 ,  C07K16/36 ,  C12P21/06 ,  C07K1/12 ,  C07K14/745

CPC分类号： C07K14/4703 ,  C07K14/745 ,  C07K16/36

摘要： A method for preparing a macromolecular protein complex (MPC) from fibrinogen in human plasma by the steps of ammonium sulfate precipitation, dialysis and gel filtration is disclosed. The isolated MPC may be degraded by contacting with trypsin. The isolated MPC inhibited fibrinolysis induced with plasminogen but not with plasmin. Elimination of the MPC by means of chondroitin sulfate A restored normal fibrinolysis. An antibody to fibrin-binding peptides which are produced by trypsin degradation of MPC, was conjugated to plasmin. The anti-MPC peptide antibody/plasmin conjugate has the capacity to bind to MPC-rich thrombus and degrade it without activation of fibrin-bound plasminogen.

摘要翻译： 公开了通过硫酸铵沉淀，透析和凝胶过滤的步骤从人血浆中的纤维蛋白原制备大分子蛋白复合物（MPC）的方法。 分离的MPC可能与胰蛋白酶接触而降解。 分离的MPC抑制纤溶酶原诱导的纤维蛋白溶解，但不抑制纤溶酶。 通过硫酸软骨素消除MPC恢复正常的纤维蛋白溶解。 通过MPC的胰蛋白酶降解产生的对纤维蛋白结合肽的抗体与纤溶酶缀合。 抗MPC肽抗体/纤溶酶偶联物具有结合富含MPC的血栓的能力，并且在没有纤维蛋白结合的纤溶酶原激活的情况下降解它。