公开(公告)号：US4657867A

公开(公告)日：1987-04-14

申请号：US667319

申请日：1984-11-01

申请人： T. Andrew Guhl ,  Luther R. Johnson

发明人： T. Andrew Guhl ,  Luther R. Johnson

IPC分类号： C12M3/00 ,  C12M1/20 ,  C12M1/32 ,  B65D51/16

CPC分类号： C12M23/12 ,  C12M23/38

摘要： A multiwell tissue culture assembly comprises a plate and a lid. The plate includes a plurality of wells for receiving liquids therein. Upstanding side walls form the outside border of the plate. A ledge is spaced inwardly of the side walls and extends around the plate between the side walls and the wells. The lid is removably positioned on the plate and includes a substantially planar cover extending over the wells. A labyrinth passage is associated with the cover, the side walls, the ledge and the wells to permit air exchange between the wells and the external environment to maintain equalibration therebetween, but to partially prevent air entrance to the wells to minimize evaporation of liquids in the wells.

摘要翻译： 多孔组织培养组件包括板和盖。 该板包括用于在其中接收液体的多个孔。 直立的侧壁形成板的外部边界。 一个凸缘与侧壁间隔开，并在侧壁和井之间围绕板延伸。 盖可拆卸地定位在板上并且包括在井上延伸的基本上平面的盖。 迷宫通道与盖子，侧壁，壁架和井相关联，以允许井与外部环境之间的空气交换以在其间保持平衡，但是部分地防止空气进入井，以使液体在 井。

公开(公告)号：US4330216A

公开(公告)日：1982-05-18

申请号：US208979

申请日：1980-11-21

申请人： Luther R. Johnson

发明人： Luther R. Johnson

IPC分类号： B01F9/08

CPC分类号： B01F9/08

摘要： A device for stirring liquid in a rotating container includes a movable stirrer adapted to be positioned inside a rotatable container and to impart stirring movement to liquid placed therein. A gravity-induced stirring mechanism is associated with the container and the stirrer for moving the stirrer with the container for only a portion of a revolution of the container when rotating. This mechanism permits the increasing gravitational force thereon due to rotation to discontinue the movement of the stirrer and cause a gravitational rotation of the stirrer in a counter-direction to that of the rotating container whereby liquid inside the container is stirred.

摘要翻译： 用于在旋转容器中搅拌液体的装置包括可移动的搅拌器，其适于定位在可旋转的容器内部并且赋予其置于其中的液体的搅拌运动。 重力引起的搅拌机构与容器和搅拌器相关联，用于在旋转时仅使容器旋转的一部分移动具有容器的搅拌器。 这种机构允许由于旋转而导致的重力增加，从而中断搅拌器的移动，并使搅拌器与旋转容器的重心相反地引起重力旋转，从而搅拌容器内部的液体。

公开(公告)号：US4317886A

公开(公告)日：1982-03-02

申请号：US176936

申请日：1980-08-11

申请人： Luther R. Johnson ,  Bruno V. Sapatino

发明人： Luther R. Johnson ,  Bruno V. Sapatino

IPC分类号： C12M3/04

CPC分类号： C12M27/12 ,  C12M25/06 ,  C12M27/20

摘要： A roller bottle for cell growth culturing comprises an outer housing defining a hollow chamber therein. At least one annular member is inside the chamber spaced a short radial distance inwardly from the housing. The space between the annular member and the housing is in fluid communication with the chamber. The surfaces of the annular members and the interior surface of the housing are adapted to grow cells thereon.

摘要翻译： 用于细胞生长培养的滚瓶包括在其中限定中空室的外壳。 至少一个环形构件在室内部，与壳体向内隔开短的径向距离。 环形构件和壳体之间的空间与腔室流体连通。 环形构件的表面和壳体的内表面适于在其上生长单元。