公开(公告)号：US20070041979A1

公开(公告)日：2007-02-22

申请号：US11506219

申请日：2006-08-18

申请人： T. Raju ,  Bernard Scallon

发明人： T. Raju ,  Bernard Scallon

IPC分类号： A61K39/395 ,  C12P21/06

CPC分类号： C07K16/00 ,  C07K2317/41 ,  C07K2317/50

摘要： Antibody preparations with substantially homogeneous and unsialylated glycoforms, such as G0 and G2, are prepared by enzymatic treatment, expression under certain conditions, use of particular host cells, and contact with serum. These antibody preparations resist cleavage by proteases, such as papain, ficin, bromolein, pepsin, a matrix metalloproteinase, such as MMP-7, neutrophil elastase (HNE), stromelysin (MMP-3) and macrophage elastase (MMP-12), and glycosylation modification enzymes. The antibody preparations with substantially homogeneous and unsialylated glycoforms and methods of testing for glycosylation in an antibody are useful in connection with characterization of antibody properties and/or in diseases or conditions characterized by an increase in protease activity.

摘要翻译： 通过酶处理，在某些条件下表达，使用特定的宿主细胞和与血清接触来制备具有基本上均相和非唾液酸化的糖形式的抗体制剂，例如G0和G2。 这些抗体制剂抵抗蛋白酶如木瓜蛋白酶，蓖麻毒素，溴林灵，胃蛋白酶，基质金属蛋白酶如MMP-7，嗜中性粒细胞弹性蛋白酶（HNE），溶基质溶解素（MMP-3）和巨噬细胞弹性蛋白酶（MMP-12）的切割，以及 糖基化修饰酶。 具有基本上均相和非唾液酸化的糖型的抗体制剂和在抗体中测试糖基化的方法可用于抗体特性表征和/或以蛋白酶活性增加为特征的疾病或病症中。