公开(公告)号：US20220372516A1

公开(公告)日：2022-11-24

申请号：US17767195

申请日：2020-10-09

申请人： TAKARA BIO INC.

发明人： Sachiko OKAMOTO ,  Izumi MAKI ,  Junichi MINENO

IPC分类号： C12N15/86 ,  C12N15/62 ,  C12N5/0783 ,  C12N15/113

摘要： Provided is a nucleic acid construct for expressing a gene of interest, the nucleic acid construct comprising, in order from 5′-terminus, each sequence of: (a) a 5′ long terminal repeat (LTR) sequence derived from a retrovirus; (b) a packaging signal sequence (IV) derived from a retrovirus; (c) a sequence or multiple cloning site of the gene of interest; (d) a post-transcriptional regulatory sequence (PRE); (e) an siRNA generating sequence which forms at least one stem-loop structure and in which RNA, which induces RNA interference in mammalian cells, is transcribed; and (f) a 3′ LTR sequence derived form a retrovirus.