公开(公告)号：US20250002868A1

公开(公告)日：2025-01-02

申请号：US18294941

申请日：2022-08-03

Applicant: TAKEDA PHARMACEUTICAL COMPANY LIMITED

Inventor： Christian Fiedler ,  Dominik MITERGRADNEGGER ,  Meinhard HASSLACHER ,  Thomas GATTERNIG ,  Daniela VASINA ,  Michael FELDHOFER ,  Renate PFANDL

IPC: C12N7/00

Abstract: The present disclosure provides for a method of purifying adeno-associated virus comprising purifying full AAV capsids from a concentrated AAV fraction or preparation comprising empty AAV capsids and full AAV capsids. The present disclosure also provides for a method of purifying adeno-associated virus comprising purifying empty AAV capsids from a concentrated AAV fraction or preparation comprising empty AAV capsids and full AAV capsids. The method utilizes one or more monovalent and one or more divalent cations to effect the separation resulting in purified full AAV capsids or empty AAV capsids.