公开(公告)号：US20210380929A1

公开(公告)日：2021-12-09

申请号：US17287214

申请日：2019-11-06

申请人： TECHNISCHE UNIVERSITÄT WIEN

发明人： Georg REISCHER ,  Roland MARTZY ,  Katharina SCHRÖDER

IPC分类号： C12N1/06 ,  C12Q1/6806

摘要： The invention relates to a method for lysing a bacterial cell comprising the steps of: providing a sample comprising the bacterial cell, and adding a lysis agent to create a lysis reaction mixture; wherein the lysis agent comprises a water-miscible ionic liquid containing the 1-Ethyl-3-methylimidazolium cation ([C2mim]). The invention further provides an apparatus for carrying out the method as well as a kit for nucleic acid amplification from a bacterial cell and a kit for nucleic acid isolation from a bacterial cell.

公开(公告)号：US20210380930A1

公开(公告)日：2021-12-09

申请号：US17287232

申请日：2019-11-06

申请人： TECHNISCHE UNIVERSITÄT WIEN

发明人： Georg REISCHER ,  Roland MARTZY ,  Katharina SCHRÖDER

IPC分类号： C12N1/06 ,  C12Q1/6806

摘要： The invention relates to a method for lysing a bacterial cell comprising the steps of: providing a sample comprising the bacterial cell, and adding a lysis agent to create a lysis reaction mixture; wherein the lysis agent comprises a water-miscible ionic liquid containing choline. The invention further provides an apparatus for carrying out the method as well as a kit for nucleic acid amplification from a bacterial cell and a kit for nucleic acid isolation from a bacterial cell.