公开(公告)号：US09988612B2

公开(公告)日：2018-06-05

申请号：US15127617

申请日：2015-03-20

Applicant: THE BOARD OF REGENTS OF THE UNIVERSITY OF TEXAS SYSTEM

Inventor： Andrew D. Ellington ,  Adam J. Meyer

IPC: C12N9/12 ,  C12P19/34 ,  C12N15/115 ,  C12N15/11

CPC classification number: C12N9/1247 ,  C12N15/111 ,  C12N15/115 ,  C12N2310/141 ,  C12N2310/16 ,  C12N2320/51 ,  C12N2330/00 ,  C12N2330/51 ,  C12P19/34 ,  C12Y207/07006

Abstract: Disclosed are T7 RNA polymerase variants with enhanced transcriptional activity. T7 RNA polymerase variants are known which have the ability to incorporate modified ribonucleotides into growing RNA molecules. However, these variants have relatively low levels of transcriptional activity. Presented herein are mutations that increase the transcriptional activity of the variants with broad substrate range.

公开(公告)号：US20170211050A1

公开(公告)日：2017-07-27

申请号：US15127617

申请日：2015-03-20

Applicant: THE BOARD OF REGENTS OF THE UNIVERSITY OF TEXAS SYSTEM

Inventor： Andrew D. Ellington ,  Adam J. Meyer

IPC: C12N9/12 ,  C12N15/115 ,  C12N15/11 ,  C12P19/34

CPC classification number: C12N9/1247 ,  C12N15/111 ,  C12N15/115 ,  C12N2310/141 ,  C12N2310/16 ,  C12N2320/51 ,  C12N2330/00 ,  C12N2330/51 ,  C12P19/34 ,  C12Y207/07006

Abstract: Disclosed are T7 RNA polymerase variants with enhanced transcriptional activity. T7 RNA polymerase variants are known which have the ability to incorporate modified ribonucleotides into growing RNA molecules. However, these variants have relatively low levels of transcriptional activity. Presented herein are mutations that increase the transcriptional activity of the variants with broad substrate range.