公开(公告)号：US20220389501A1

公开(公告)日：2022-12-08

申请号：US17761785

申请日：2020-09-18

申请人： THE UNIVERSITY OF BIRMINGHAM

发明人： Robert NEELY ,  Francisco FERNANDEZ-TRILLO ,  Jack KENNEFICK ,  Andrew WILKINSON

IPC分类号： C12Q1/6869 ,  C12Q1/6806

摘要： The present invention relates to a method for analyzing DNA including forming labeled DNA fragments by cleaving genomic DNA into DNA fragments, selectively functionalizing any non-methylated CpG sites present in the DNA with a linker including a hydrolyzable moiety, and attaching a label to the linker. The method further includes the step of separating the labeled DNA fragments from any non-labeled DNA fragments, hydrolyzing the hydrolyzable moiety of the linker of the separated labeled DNA fragments so as to release the DNA fragments from the label, and sequencing the released DNA fragments.

公开(公告)号：US20220340946A1

公开(公告)日：2022-10-27

申请号：US17761817

申请日：2020-09-18

申请人： THE UNIVERSITY OF BIRMINGHAM

发明人： Robert NEELY ,  Francisco FERNANDEZ-TRILLO ,  Elodie JAGO ,  Andrew WILKINSON

IPC分类号： C12P19/34

摘要： A method of reversibly labeling a biomolecule including providing a linker molecule (11) having a first functional group (LG) with a reactive center, a second functional group (FG) with a reactive center, and a cleavable, e.g. hydrolyzable, moiety (A-B-C). The method further includes forming a covalent bond between the biomolecule (10) and the reactive center of the first functional group (LG), forming a covalent bond between a first label (L1) and the reactive center of the second functional group (FG), cleaving the cleavable moiety (A-B-C), e.g. hydrolyzing the hydrolyzable moiety, of the linker molecule (11) to remove the first label (L1) and to form a third functional group (W) with a reactive center, and forming a covalent bond between a further molecule and the reactive center of the third functional group (W) to reform the cleavable moiety, e.g. hydrolyzable moiety (A-B-C).