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公开(公告)号：US20060177846A1
公开(公告)日：2006-08-10
申请号：US11289378
申请日：2005-11-30
摘要： A technology that allows a consensus sequence targeted for primer design to be easily displayed is provided. In sequence data of analysis targets, a consensus nucleotide sequence is generated by performing multiple sequence alignment based on nucleotide sequence, and a consensus amino acid sequence is generated by performing multiple sequence alignment based on amino acid sequence, followed by generating additional consensus nucleotide sequence by reverse translation of the consensus amino acid sequence. In other words, two consensus sequences consisting of the consensus sequence based on nucleotide sequence and the consensus sequence based on amino acid sequence are generated. One of these two consensus sequences can be chosen as a target for primers on a screen to input parameters for primer design.
摘要翻译： 提供了一种能够容易地显示针对引物设计的共识序列的技术。 在分析目标的序列数据中，通过基于核苷酸序列进行多个序列比对来产生共有核苷酸序列，并且通过基于氨基酸序列进行多个序列比对来产生共有氨基酸序列，随后通过 共同氨基酸序列的反向翻译。 换句话说，产生由基于核苷酸序列的共有序列和基于氨基酸序列的共有序列组成的两个共有序列。 可以选择这两个共有序列之一作为屏幕上的引物的目标，以输入引物设计的参数。
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公开(公告)号：US07698069B2
公开(公告)日：2010-04-13
申请号：US11185892
申请日：2005-07-21
申请人： Takamune Yamamoto
发明人： Takamune Yamamoto
IPC分类号： G01N33/48
摘要： A method for designing a primer for real-time PCR comprises retrieving a primer set using a known primer search algorithm and based on primer design conditions, visually displaying the retrieved primer set together with its splicing information and base sequence, narrowing the primer set using narrowing conditions, and visually displaying a narrowed primer set together with its splicing information and base sequence.
摘要翻译： 一种用于实时PCR设计引物的方法，包括使用已知的引物搜索算法和基于引物设计条件检索引物组，在视觉上显示所检索的引物组及其剪接信息和碱基序列，使用缩小来缩小引物组 条件，并且可视地显示缩小的引物组及其剪接信息和碱基序列。
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公开(公告)号：US20060047442A1
公开(公告)日：2006-03-02
申请号：US11185892
申请日：2005-07-21
申请人： Takamune Yamamoto
发明人： Takamune Yamamoto
IPC分类号： G06F19/00
摘要： A method for designing a primer for real-time PCR comprises retrieving a primer set using a known primer search algorithm and based on primer design conditions, visually displaying the retrieved primer set together with its splicing information and base sequence, narrowing the primer set using narrowing conditions, and visually displaying a narrowed primer set together with its splicing information and base sequence.
摘要翻译： 一种用于实时PCR设计引物的方法，包括使用已知的引物搜索算法和基于引物设计条件检索引物组，在视觉上显示所检索的引物组及其剪接信息和碱基序列，使用缩小来缩小引物组 条件，并且可视地显示缩小的引物组及其剪接信息和碱基序列。
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