公开(公告)号：US20240309341A1

公开(公告)日：2024-09-19

申请号：US18660667

申请日：2024-05-10

申请人： Takara Bio Inc.

发明人： Takashi UEMORI ,  Hiroyuki Matsumoto ,  Kensuke Saito ,  Miwa Akitomo

IPC分类号： C12N9/12

CPC分类号： C12N9/1252 ,  C12Y207/07007

摘要： Provided are: a DNA polymerase mutant having reverse transcriptase activity, the DNA polymerase mutant including a sequence consisting of twelve specific amino acids A1-A12, wherein the DNA polymerase mutant having reverse transcriptase activity is characterized in that the A3 and/or A10 amino acid is substituted by a basic amino acid residue different from that prior to the introduction of mutation; a kit and a composition including the DNA polymerase; a method for producing the DNA polymerase; and a method for modifying an existing DNA polymerase having reverse transcriptase activity.

公开(公告)号：US12018294B2

公开(公告)日：2024-06-25

申请号：US17257986

申请日：2019-07-03

申请人： TAKARA BIO INC.

发明人： Takashi Uemori ,  Hiroyuki Matsumoto ,  Kensuke Saito ,  Miwa Akitomo

IPC分类号： C12N9/12

CPC分类号： C12N9/1252 ,  C12Y207/07007

摘要： Provided are: a DNA polymerase mutant having reverse transcriptase activity, the DNA polymerase mutant including a sequence consisting of twelve specific amino acids A1-A12, wherein the DNA polymerase mutant having reverse transcriptase activity is characterized in that the A3 and/or A10 amino acid is substituted by a basic amino acid residue different from that prior to the introduction of mutation; a kit and a composition including the DNA polymerase; a method for producing the DNA polymerase; and a method for modifying an existing DNA polymerase having reverse transcriptase activity.