公开(公告)号：US20050265979A1

公开(公告)日：2005-12-01

申请号：US11082055

申请日：2005-03-17

申请人： Takeshi Aoki ,  Mitsuo Kusano ,  Yoshinori Shimizu ,  Tomotake Koizumi ,  Daisuke Yasuda ,  Yoshihiko Izumida ,  Noriyuki Murai ,  Zhenghao Jin ,  Yasuna Kobayashi ,  Hirohisa Kato ,  Luchun Hua

发明人： Takeshi Aoki ,  Mitsuo Kusano ,  Yoshinori Shimizu ,  Tomotake Koizumi ,  Daisuke Yasuda ,  Yoshihiko Izumida ,  Noriyuki Murai ,  Zhenghao Jin ,  Yasuna Kobayashi ,  Hirohisa Kato ,  Luchun Hua

IPC分类号： A01N1/02 ,  A61K45/00

CPC分类号： A01N1/0221 ,  A01N1/02

摘要： A method is disclosed for cryopreserving living animal cells in immunoisolation membranes, including: (1) cutting out a living organ from an animal, (2) digesting the cutout organ into the discrete living animal cells and separating the discrete cells, (3) suspending the separated cells in a solution of sodium chloride containing sodium alginate and collagen, (4) forming microcapsules of the living animal cells by using the resulting suspension, (5) forming immunoisolation membranes around outer surfaces of the microcapsules of the living animal cells by covering the outer surfaces with alginate-(poly-L-lysine) and thereby obtaining the living animal cells enclosed in the immunoisolation membranes, (6) suspending the resulting living animal cells enclosed in the immunocapsules in a cell damage-preventing solution, and (7) immediately with liquid nitrogen.

摘要翻译： 公开了一种在免疫隔离膜中冷冻保存活体动物细胞的方法，包括：（1）从动物切出活体器官，（2）将切除的器官消化到离散的活细胞中并分离离散的细胞，（3）悬浮 在含有藻酸钠和胶原的氯化钠溶液中分离的细胞，（4）通过使用所得到的悬浮液形成活性动物细胞的微胶囊，（5）通过覆盖活体动物细胞的微胶囊的外表面形成免疫隔离膜 具有海藻酸盐 - （聚-L-赖氨酸）的外表面，从而获得封闭在免疫隔离膜中的活体动物细胞，（6）将封闭在免疫胶囊中的所得活体动物细胞悬浮于细胞损伤预防溶液中，和（7 ）立即用液氮。

公开(公告)号：US07553612B2

公开(公告)日：2009-06-30

申请号：US11082055

申请日：2005-03-17

申请人： Takeshi Aoki ,  Mitsuo Kusano ,  Yoshinori Shimizu ,  Tomotake Koizumi ,  Daisuke Yasuda ,  Yoshihiko Izumida ,  Noriyuki Murai ,  Zhenghao Jin ,  Yasuna Kobayashi ,  Hirohisa Kato ,  Luchun Hua

发明人： Takeshi Aoki ,  Mitsuo Kusano ,  Yoshinori Shimizu ,  Tomotake Koizumi ,  Daisuke Yasuda ,  Yoshihiko Izumida ,  Noriyuki Murai ,  Zhenghao Jin ,  Yasuna Kobayashi ,  Hirohisa Kato ,  Luchun Hua

IPC分类号： A01N1/02

CPC分类号： A01N1/0221 ,  A01N1/02

摘要： A method is disclosed for cryopreserving living animal cells in immunoisolation membranes, including: (1) cutting out a living organ from an animal, (2) digesting the cutout organ into the discrete living animal cells and separating the discrete cells, (3) suspending the separated cells in a solution of sodium chloride containing sodium alginate and collagen, (4) forming microcapsules of the living animal cells by using the resulting suspension, (5) forming immunoisolation membranes around outer surfaces of the microcapsules of the living animal cells by covering the outer surfaces with alginate-(poly-L-lysine) and thereby obtaining the living animal cells enclosed in the immunoisolation membranes, (6) suspending the resulting living animal cells enclosed in the immunocapsules in a cell damage-preventing solution, and (7) immediately freezing the thus obtained suspension with liquid nitrogen.

摘要翻译： 公开了一种在免疫隔离膜中冷冻保存活体动物细胞的方法，包括：（1）从动物切出活体器官，（2）将切除的器官消化到离散的活细胞中并分离离散的细胞，（3）悬浮 在含有藻酸钠和胶原的氯化钠溶液中分离的细胞，（4）通过使用所得到的悬浮液形成活性动物细胞的微胶囊，（5）通过覆盖活体动物细胞的微胶囊的外表面形成免疫隔离膜 具有海藻酸盐 - （聚-L-赖氨酸）的外表面，从而获得封闭在免疫隔离膜中的活体动物细胞，（6）将封闭在免疫胶囊中的所得活体动物细胞悬浮于细胞损伤预防溶液中，和（7 ）立即用液氮将如此得到的悬浮液冷冻。