公开(公告)号：US20090131271A1

公开(公告)日：2009-05-21

申请号：US11914702

申请日：2006-05-16

申请人： Takuro Niidome ,  Fumio Wada ,  Yoshiki Katayama ,  Kosuke Yakumaru ,  Takahito Kawano ,  Shuzo Yamashita

发明人： Takuro Niidome ,  Fumio Wada ,  Yoshiki Katayama ,  Kosuke Yakumaru ,  Takahito Kawano ,  Shuzo Yamashita

IPC分类号： C40B30/06 ,  C12N15/87 ,  C40B60/12 ,  C12M1/00

CPC分类号： C12N15/87

摘要： In a method for delivering a nucleic acid of the invention, a nucleic acid is introduced into a cell by pressing the nucleic acid supported on a surface of a solid substrate against the cell. According to the method, the nucleic acid can be delivered into the cell simply at a low cost without placing a heavy burden on the cell at a high nucleic acid delivery efficiency. By allowing the surface of the solid substrate to support the nucleic acid in the form of a complex with a polyamine or a cationic lipid and pressing it against the cell, the introduction efficiency into the cell can be further improved. In the invention, by using a nucleic acid useful for gene therapy as the nucleic acid, a high-efficiency device for gene therapy can be obtained.

摘要翻译： 在递送本发明的核酸的方法中，通过将负载在固体基质表面上的核酸压在细胞上，将核酸引入细胞。 根据该方法，可以简单地以低成本将核酸递送到细胞中，而不会以高核酸输送效率对细胞施加沉重的负担。 通过使固体基质的表面以与多胺或阳离子脂质的复合物的形式支持核酸并将其压在细胞上，可以进一步提高进入细胞的引入效率。 在本发明中，通过使用用于基因治疗的核酸作为核酸，可以获得用于基因治疗的高效装置。