Two wave length, scanning and integration type method of
microspectrophotometrically measuring the light absorbancy of a
specimen and microspectrophotometer for automatically carrying out the
method of measurement
    2.
    发明授权
    Two wave length, scanning and integration type method of microspectrophotometrically measuring the light absorbancy of a specimen and microspectrophotometer for automatically carrying out the method of measurement 失效
    两种波长,扫描和积分型微观分光光度法测量样品和显微分光光度计的光吸收率,用于自动执行测量方法

    公开(公告)号:US4012146A

    公开(公告)日:1977-03-15

    申请号:US426240

    申请日:1973-12-19

    IPC分类号: G01N21/27 G01N21/31 G01J3/42

    CPC分类号: G01N21/314

    摘要: A two wave length, scanning and integration type method of microspectrophotometrically measuring the light absorbancy of a specimen dyed with a coloring matter comprising scanning the specimen by light spots having two different wave lengths and integrating electric signals converted from the light spots traversing the specimen. The two different wave lengths .lambda..sub.1 and .lambda..sub.2 are selected such that at the wave length .lambda..sub.1 the light absorbancy of the absorption spectrum of the coloring matter is maximum and that at the wave length .lambda..sub.2 the light absorbancy is zero and the wave length .lambda..sub.2 is deviated from the absorption spectrum and located near the wave length .lambda..sub.1. A true light absorbancy in proportion to the amount of light absorbing substance at that part of the specimen which is to be measured is obtained by subtracting the light absorbancy produced by the light loss not peculiar to the specimen and caused by the light diffusion due to light reflection, refraction and diffraction in the specimen from the light absorbancy peculiar to the specimen. A two wave length, scanning and integration type microspectrophotometer for automatically carrying out the method of measurement is also disclosed.

    摘要翻译: 一种二波长扫描和一体化方法,用于显微分光测量用色素染色的试样的光吸收,包括用两个不同波长的光斑扫描试样并积分从穿过试样的光斑转换的电信号。 选择两种不同的波长λ1和λ2,使得在波长λ1处,色素的吸收光谱的光吸收最大,而在波长λ2处的光吸收为零,波长 λ2偏离吸收光谱并位于波长λ1附近。与要测量的该部分的光吸收物质的量成比例的真实光吸收通过减去由待测量的样品产生的光吸收 由于样品中由于样品特有的光吸收而导致的光反射,折射和衍射而引起的光扩散而导致的样品特有的光损失。 还公开了一种用于自动执行测量方法的两波长扫描和积分型显微分光光度计。