公开(公告)号：US12139750B2

公开(公告)日：2024-11-12

申请号：US17168950

申请日：2021-02-05

Applicant: The Chinese University of Hong Kong

Inventor： Yuk-Ming Dennis Lo ,  Rossa Wai Kwun Chiu ,  Kwan Chee Chan ,  Peiyong Jiang ,  Suk Hang Cheng ,  Cheuk Yin Yu ,  Yee Ting Cheung ,  Wenlei Peng

IPC: C12Q1/6827 ,  C12Q1/6806 ,  C12Q1/6881 ,  C12Q1/6883 ,  G16B20/20 ,  G16B30/10 ,  G16B40/00

Abstract: Methods and systems described herein involve using long cell-free DNA fragments to analyze a biological sample from a pregnant subject. The status of methylated CpG sites and single nucleotide polymorphisms (SNPs) is often used to analyze DNA fragments of a biological sample. A CpG site and a SNP are typically separated from the nearest CpG site or SNP by hundreds or thousands of base pairs. Finding two or more consecutive CpG sites or SNPs on most cell-free DNA fragments is improbable or impossible. Cell-free DNA fragments longer than 600 bp may include multiple CpG sites and/or SNPs. The presence of multiple CpG sites and/or SNPs on long cell-free DNA fragments may allow for analysis than with short cell-free DNA fragments alone. The long cell-free DNA fragments can be used to identify a tissue of origin and/or to provide information on a fetus in a pregnant female.

公开(公告)号：US20220275433A1

公开(公告)日：2022-09-01

申请号：US17743315

申请日：2022-05-12

Applicant: The Chinese University of Hong Kong

Inventor： Yuk-Ming Dennis Lo ,  Rossa Wai Kwun Chiu ,  Kwan Chee Chan ,  Peiyong Jiang ,  Suk Hang Cheng ,  Cheuk Yin Yu ,  Yee Ting Cheung ,  Wenlei Peng

IPC: C12Q1/6827 ,  C12Q1/6806 ,  G16B30/10 ,  G16B20/20 ,  G16B40/00 ,  C12Q1/6881 ,  C12Q1/6883

Abstract: Methods and systems described herein involve using long cell-free DNA fragments to analyze a biological sample from a pregnant subject. The status of methylated CpG sites and single nucleotide polymorphisms (SNPs) is often used to analyze DNA fragments of a biological sample. A CpG site and a SNP are typically separated from the nearest CpG site or SNP by hundreds or thousands of base pairs. Finding two or more consecutive CpG sites or SNPs on most cell-free DNA fragments is improbable or impossible. Cell-free DNA fragments longer than 600 bp may include multiple CpG sites and/or SNPs. The presence of multiple CpG sites and/or SNPs on long cell-free DNA fragments may allow for analysis than with short cell-free DNA fragments alone. The long cell-free DNA fragments can be used to identify a tissue of origin and/or to provide information on a fetus in a pregnant female.

公开(公告)号：US20210254142A1

公开(公告)日：2021-08-19

申请号：US17196765

申请日：2021-03-09

Applicant: The Chinese University of Hong Kong

Inventor： Yuk-Ming Dennis Lo ,  Rossa Wai Kwun Chiu ,  Kwan Chee Chan ,  Peiyong Jiang ,  Suk Hang Cheng ,  Cheuk Yin Yu ,  Yee Ting Cheung ,  Wenlei Peng

IPC: C12Q1/6827 ,  C12Q1/6806

Abstract: Methods and systems described herein involve using long cell-free DNA fragments to analyze a biological sample from a pregnant subject. The status of methylated CpG sites and single nucleotide polymorphisms (SNPs) is often used to analyze DNA fragments of a biological sample. A CpG site and a SNP are typically separated from the nearest CpG site or SNP by hundreds or thousands of base pairs. Finding two or more consecutive CpG sites or SNPs on most cell-free DNA fragments is improbable or impossible. Cell-free DNA fragments longer than 600 bp may include multiple CpG sites and/or SNPs. The presence of multiple CpG sites and/or SNPs on long cell-free DNA fragments may allow for analysis than with short cell-free DNA fragments alone. The long cell-free DNA fragments can be used to identify a tissue of origin and/or to provide information on a fetus in a pregnant female.

公开(公告)号：US20210265007A1

公开(公告)日：2021-08-26

申请号：US17168950

申请日：2021-02-05

Applicant: The Chinese University of Hong Kong

Inventor： Yuk-Ming Dennis Lo ,  Rossa Wai Kwun Chiu ,  Kwan Chee Chan ,  Peiyong Jiang ,  Suk Hang Cheng ,  Cheuk Yin Yu ,  Yee Ting Cheung ,  Wenlei Peng

IPC: G16B20/20 ,  C12Q1/6881 ,  C12Q1/6883 ,  G16B30/10 ,  G16B40/00

Abstract: Methods and systems described herein involve using long cell-free DNA fragments to analyze a biological sample from a pregnant subject. The status of methylated CpG sites and single nucleotide polymorphisms (SNPs) is often used to analyze DNA fragments of a biological sample. A CpG site and a SNP are typically separated from the nearest CpG site or SNP by hundreds or thousands of base pairs. Finding two or more consecutive CpG sites or SNPs on most cell-free DNA fragments is improbable or impossible. Cell-free DNA fragments longer than 600 bp may include multiple CpG sites and/or SNPs. The presence of multiple CpG sites and/or SNPs on long cell-free DNA fragments may allow for analysis than with short cell-free DNA fragments alone. The long cell-free DNA fragments can be used to identify a tissue of origin and/or to provide information on a fetus in a pregnant female.

公开(公告)号：US11591642B2

公开(公告)日：2023-02-28

申请号：US17743315

申请日：2022-05-12

Applicant: The Chinese University of Hong Kong

Inventor： Yuk-Ming Dennis Lo ,  Rossa Wai Kwun Chiu ,  Kwan Chee Chan ,  Peiyong Jiang ,  Suk Hang Cheng ,  Cheuk Yin Yu ,  Yee Ting Cheung ,  Wenlei Peng

IPC: C12Q1/68 ,  C12Q1/6827 ,  C12Q1/6806 ,  G16B30/10 ,  G16B20/20 ,  G16B40/00 ,  C12Q1/6881 ,  C12Q1/6883

Abstract: Methods and systems described herein involve using long cell-free DNA fragments to analyze a biological sample from a pregnant subject. The status of methylated CpG sites and single nucleotide polymorphisms (SNPs) is often used to analyze DNA fragments of a biological sample. A CpG site and a SNP are typically separated from the nearest CpG site or SNP by hundreds or thousands of base pairs. Finding two or more consecutive CpG sites or SNPs on most cell-free DNA fragments is improbable or impossible. Cell-free DNA fragments longer than 600 bp may include multiple CpG sites and/or SNPs. The presence of multiple CpG sites and/or SNPs on long cell-free DNA fragments may allow for analysis than with short cell-free DNA fragments alone. The long cell-free DNA fragments can be used to identify a tissue of origin and/or to provide information on a fetus in a pregnant female.

公开(公告)号：US11371084B2

公开(公告)日：2022-06-28

申请号：US17196765

申请日：2021-03-09

Applicant: The Chinese University of Hong Kong

Inventor： Yuk-Ming Dennis Lo ,  Rossa Wai Kwun Chiu ,  Kwan Chee Chan ,  Peiyong Jiang ,  Suk Hang Cheng ,  Cheuk Yin Yu ,  Yee Ting Cheung ,  Wenlei Peng

IPC: C12Q1/68 ,  C12Q1/6827 ,  C12Q1/6806 ,  G16B30/10 ,  G16B20/20 ,  G16B40/00 ,  C12Q1/6881 ,  C12Q1/6883

Abstract: Methods and systems described herein involve using long cell-free DNA fragments to analyze a biological sample from a pregnant subject. The status of methylated CpG sites and single nucleotide polymorphisms (SNPs) is often used to analyze DNA fragments of a biological sample. A CpG site and a SNP are typically separated from the nearest CpG site or SNP by hundreds or thousands of base pairs. Finding two or more consecutive CpG sites or SNPs on most cell-free DNA fragments is improbable or impossible. Cell-free DNA fragments longer than 600 bp may include multiple CpG sites and/or SNPs. The presence of multiple CpG sites and/or SNPs on long cell-free DNA fragments may allow for analysis than with short cell-free DNA fragments alone. The long cell-free DNA fragments can be used to identify a tissue of origin and/or to provide information on a fetus in a pregnant female.