公开(公告)号：US09297806B2

公开(公告)日：2016-03-29

申请号：US13956879

申请日：2013-08-01

申请人： The Johns Hopkins University

发明人： Srinivasan Yegnasubramanian ,  Michael Christoph Haffner ,  Alcides Chaux ,  William G. Nelson ,  Angelo M. DeMarzo

IPC分类号： G01N33/53 ,  G01N33/574

CPC分类号： G01N33/57496 ,  G01N2800/50

摘要： The present invention relates to the field of cancer. More specifically, the present invention provides methods and compositions useful for diagnosing or predicting cancer in a patient. In one embodiment, a method for identifying a patient as having cancer comprises the steps of (a) providing a formalin-fixed, paraffin-embedded or fresh frozen sample of patient tissue; (b) steaming the sample in antigen retrieval buffer; (c) incubating the sample in hydrochloric acid (HCl); (d) incubating the sample with an affinity reagent specific for 5hmC under conditions to form a complex between the affinity reagent and 5-hydroxymethylcytosine (5hmC) present in the sample; (e) detecting the complexes formed between 5hmC and the affinity reagent with secondary detection reagents; (f) quantifying 5hmC levels; and (g) identifying the patient as having cancer if the 5hmC levels in the sample are reduced as compared to a control.

摘要翻译： 本发明涉及癌症领域。 更具体地，本发明提供了可用于诊断或预测患者中的癌症的方法和组合物。 在一个实施方案中，用于鉴定患者具有癌症的方法包括以下步骤：（a）提供患有组织的福尔马林固定，石蜡包埋或新鲜冷冻的样品; （b）在抗原检索缓冲液中蒸煮样品; （c）将样品在盐酸（HCl）中孵育; （d）在条件下用特异于5hmC的亲和试剂培养样品，以形成样品中存在的亲和试剂和5-羟甲基胞嘧啶（5hmC）之间的复合物; （e）用二级检测试剂检测在5hmC与亲和试剂之间形成的复合物; （f）量化5hmC水平; 如果与对照相比，如果样品中的5hmC水平降低，则鉴定患者具有癌症。

公开(公告)号：US20140038183A1

公开(公告)日：2014-02-06

申请号：US13956879

申请日：2013-08-01

申请人： The Johns Hopkins University

发明人： Srinivasan Yegnasubramanian ,  Michael Christoph Haffner ,  Alcides Chaux ,  William G. Nelson ,  Angelo M. DeMarzo

IPC分类号： G01N33/574

CPC分类号： G01N33/57496 ,  G01N2800/50

摘要： The present invention relates to the field of cancer. More specifically, the present invention provides methods and compositions useful for diagnosing or predicting cancer in a patient. In one embodiment, a method for identifying a patient as having cancer comprises the steps of (a) providing a formalin-fixed, paraffin-embedded or fresh frozen sample of patient tissue; (b) steaming the sample in antigen retrieval buffer; (c) incubating the sample in hydrochloric acid (HCl); (d) incubating the sample with an affinity reagent specific for 5hmC under conditions to form a complex between the affinity reagent and 5-hydroxymethylcytosine (5hmC) present in the sample; (e) detecting the complexes formed between 5hmC and the affinity reagent with secondary detection reagents; (f) quantifying 5hmC levels; and (g) identifying the patient as having cancer if the 5hmC levels in the sample are reduced as compared to a control.

摘要翻译： 本发明涉及癌症领域。 更具体地，本发明提供了可用于诊断或预测患者中的癌症的方法和组合物。 在一个实施方案中，用于鉴定患者具有癌症的方法包括以下步骤：（a）提供患有组织的福尔马林固定，石蜡包埋或新鲜冷冻的样品; （b）在抗原检索缓冲液中蒸煮样品; （c）将样品在盐酸（HCl）中孵育; （d）在条件下用特异于5hmC的亲和试剂培养样品，以形成样品中存在的亲和试剂和5-羟甲基胞嘧啶（5hmC）之间的复合物; （e）用二级检测试剂检测在5hmC与亲和试剂之间形成的复合物; （f）量化5hmC水平; 如果与对照相比，如果样品中的5hmC水平降低，则鉴定患者具有癌症。