公开(公告)号：US10915729B2

公开(公告)日：2021-02-09

申请号：US16277128

申请日：2019-02-15

申请人： The Regents of The University of Michigan

发明人： Ivaylo Dinov ,  Brian D. Athey ,  David S. Dilworth ,  Ari Allyn-Feuer ,  Alexandr Kalinin ,  Alex S. Ade

IPC分类号： G06K9/00 ,  G06K9/46 ,  G06K9/62 ,  G01N1/30 ,  G06T7/62 ,  G06T7/50

摘要： The ability to automate the processes of specimen collection, image acquisition, data pre-processing, computation of derived biomarkers, modeling, classification and analysis can significantly impact clinical decision-making and fundamental investigation of cell deformation. This disclosure combine 3D cell nuclear shape modeling by robust smooth surface reconstruction and extraction of shape morphometry measure into a highly parallel pipeline workflow protocol for end-to-end morphological analysis of thousands of nuclei and nucleoli in 3D. This approach allows efficient and informative evaluation of cell shapes in the imaging data and represents a reproducible technique that can be validated, modified, and repurposed by the biomedical community. This facilitates result reproducibility, collaborative method validation, and broad knowledge dissemination.