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公开(公告)号:US20210109086A1
公开(公告)日:2021-04-15
申请号:US16885028
申请日:2020-05-27
申请人: The Scripps Research Institute , The Board of Trustees of The Leland Stanford Junior University
摘要: The present invention provides methods for diagnosing lung cancer in a subject comprising (a) generating circulating tumor cell (CTC) data from a blood sample obtained from the subject based on a direct analysis comprising immunofluorescent staining and morphological characteristics of nucleated cells in the sample, wherein CTCs are identified in context of surrounding nucleated cells based on a combination of the immunofluorescent staining and morphological characteristics; (b) obtaining clinical data for the subject; (c) combining the CTC data with the clinical data to diagnose lung cancer in the subject.
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公开(公告)号:US20210311025A1
公开(公告)日:2021-10-07
申请号:US17345968
申请日:2021-06-11
发明人: Fei Liu , Utkan Demirci , Sanjiv Sam Gambhir , Viswam S. Nair
摘要: A device (“the ExoTIC device”) for the isolation of extracellular vesicles from an extracellular vesicle-containing sample in which the sample is flowed through a membrane in a flow chamber to capture and purify the extracellular vesicles on the membrane. The extracellular vesicles may be washed and collected and utilized in any one of a number of ways including, but not limited to, identifying biomarkers of a disease, identifying the presence of a biomarker in a patient to determine whether a patient has a disease, and therapeutically treating existing diseases by re-introducing the extracellular vesicles, potentially modified, back into a body.
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公开(公告)号:US11073511B2
公开(公告)日:2021-07-27
申请号:US16073577
申请日:2017-02-01
发明人: Fei Liu , Utkan Demirci , Sanjiv Sam Gambhir , Viswam S. Nair
摘要: A device (“the ExoTIC device”) for the isolation of extracellular vesicles from an extracellular vesicle-containing sample in which the sample is flowed through a membrane in a flow chamber to capture and purify the extracellular vesicles on the membrane. The extracellular vesicles may be washed and collected and utilized in any one of a number of ways including, but not limited to, identifying biomarkers of a disease, identifying the presence of a biomarker in a patient to determine whether a patient has a disease, and therapeutically treating existing diseases by re-introducing the extracellular vesicles, potentially modified, back into a body.
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公开(公告)号:US20180002760A2
公开(公告)日:2018-01-04
申请号:US15133996
申请日:2016-04-20
发明人: Seung-min Park , Dawson Wong , Chin Chun Ooi , Sanjiv Sam Gambhir , Viswam S. Nair , Shan X. Wang
IPC分类号: C12Q1/68
CPC分类号: C12Q1/6886 , C12Q1/686 , C12Q2600/158 , G06F17/30132 , C12Q2521/107 , C12Q2537/143
摘要: A method for identification of circulating tumor cells (CTCs) in a blood sample uses magnetic enrichment and a nanowell assay. The CTCs are magnetically labeled with cancer cell markers conjugated to magnetic nanoparticles and then separated by passing the blood sample through a magnetic sifter. The enriched CTCs are then loaded into a microfluidic single-cell molecular assay comprising an array of 25,600 or more nanowells, each containing at most a single one of the CTCs. Using multiple fluorescent gene markers, simultaneous multiple-color multiplexed gene expression of the CTCs is performed, preferably using RT-PCR. Images of fluorescence signals from individual nanowells are analyzed to identify CTCs.
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公开(公告)号:US10167515B2
公开(公告)日:2019-01-01
申请号:US15133996
申请日:2016-04-20
发明人: Seung-min Park , Dawson Wong , Chin Chun Ooi , Sanjiv Sam Gambhir , Viswam S. Nair , Shan X. Wang
IPC分类号: C12Q1/6886 , C12Q1/686 , G06F17/30
摘要: A method for identification of circulating tumor cells (CTCs) in a blood sample uses magnetic enrichment and a nanowell assay. The CTCs are magnetically labeled with cancer cell markers conjugated to magnetic nanoparticles and then separated by passing the blood sample through a magnetic sifter. The enriched CTCs are then loaded into a microfluidic single-cell molecular assay comprising an array of 25,600 or more nanowells, each containing at most a single one of the CTCs. Using multiple fluorescent gene markers, simultaneous multiple-color multiplexed gene expression of the CTCs is performed, preferably using RT-PCR. Images of fluorescence signals from individual nanowells are analyzed to identify CTCs.
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