公开(公告)号：US20140056936A1

公开(公告)日：2014-02-27

申请号：US13924275

申请日：2013-06-21

申请人： The United States Government as represented by the Department of Veterans Affairs ,  Oregon Health & Science University

发明人： Halina Offner ,  Patricia D. Hurn ,  Arthur A. Vandenbark ,  Gregory G. Burrows

IPC分类号： A61K39/00

CPC分类号： A61K39/385 ,  A61K39/00 ,  A61K39/0005 ,  A61K39/395 ,  A61K45/06 ,  A61K2039/605 ,  A61K2039/627

摘要： Two-domain MHC polypeptides are useful for modulating activities of antigen-specific T-cells, including for modulating pathogenic potential and effects of antigen-specific T-cells. Exemplary MHC class II-based recombinant T-cell ligands (RTLs) of the invention include covalently linked β1 and α1 domains, and MHC class I-based molecules that comprise covalently linked α1 and α2 domains. These polypeptides may also include covalently linked antigenic determinants, toxic moieties, and/or detectable labels. The disclosed polypeptides can be used to target antigen-specific T-cells, and are useful, among other things, to detect and purify antigen-specific T-cells, to induce or activate T-cells, to modulate T-cell activity, including by regulatory switching of T-cell cytokine and adhesion molecule expression, to treat conditions mediated by antigen-specific T-cells, including treatment and/or prevention of central nervous system damage relating to stroke.

摘要翻译： 双域MHC多肽可用于调节抗原特异性T细胞的活性，包括调节致病潜力和抗原特异性T细胞的作用。 本发明的示例性基于MHC II类的重组T细胞配体（RTL）包括共价连接的β1和α1结构域，以及包含共价连接的α1和α2结构域的基于MHC I类的分子。 这些多肽还可以包括共价连接的抗原决定簇，毒性部分和/或可检测的标记。 所公开的多肽可用于靶向抗原特异性T细胞，并且除其他外，可用于检测和纯化抗原特异性T细胞，诱导或激活T细胞以调节T细胞活性，包括 通过T细胞因子和粘附分子表达的调节转换来治疗由抗原特异性T细胞介导的病症，包括治疗和/或预防与中风有关的中枢神经系统损伤。

公开(公告)号：US20150343055A1

公开(公告)日：2015-12-03

申请号：US14732145

申请日：2015-06-05

申请人： Oregon Health & Science University ,  The United States Government as represented by the Department of Veterans Affairs

发明人： Halina Offner ,  Patricia D. Hurn ,  Arthur A. Vandenbark ,  Gregory G. Burrows

IPC分类号： A61K39/385 ,  A61K45/06

CPC分类号： A61K39/385 ,  A61K39/00 ,  A61K39/0005 ,  A61K39/395 ,  A61K45/06 ,  A61K2039/605 ,  A61K2039/627

摘要： Two-domain MHC polypeptides are useful for modulating activities of antigen-specific T-cells, including for modulating pathogenic potential and effects of antigen-specific T-cells. Exemplary MHC class II-based recombinant T-cell ligands (RTLs) of the invention include covalently linked β1 and α1 domains, and MHC class I-based molecules that comprise covalently linked α1 and α2 domains. These polypeptides may also include covalently linked antigenic determinants, toxic moieties, and/or detectable labels. The disclosed polypeptides can be used to target antigen-specific T-cells, and are useful, among other things, to detect and purify antigen-specific T-cells, to induce or activate T-cells, to modulate T-cell activity, including by regulatory switching of T-cell cytokine and adhesion molecule expression, to treat conditions mediated by antigen-specific T-cells, including treatment and/or prevention of central nervous system damage relating to stroke.

摘要翻译： 双域MHC多肽可用于调节抗原特异性T细胞的活性，包括调节致病潜力和抗原特异性T细胞的作用。 本发明的示例性基于MHC II类的重组T细胞配体（RTL）包括共价连接的1和α1结构域，以及包含共价连接的α1和α2结构域的基于MHC I类的分子。 这些多肽还可以包括共价连接的抗原决定簇，毒性部分和/或可检测的标记。 所公开的多肽可用于靶向抗原特异性T细胞，并且除其他外，可用于检测和纯化抗原特异性T细胞，诱导或激活T细胞以调节T细胞活性，包括 通过T细胞因子和粘附分子表达的调节转换来治疗由抗原特异性T细胞介导的病症，包括治疗和/或预防与中风有关的中枢神经系统损伤。

公开(公告)号：US09050279B2

公开(公告)日：2015-06-09

申请号：US13924275

申请日：2013-06-21

申请人： Oregon Health & Science University ,  The United States of America as represented by the Department of Veterans Affairs

发明人： Halina Offner ,  Patricia D. Hurn ,  Arthur A. Vandenbark ,  Gregory G. Burrows

IPC分类号： A61K39/385 ,  A61K39/00 ,  A61K39/395 ,  A61K38/16

CPC分类号： A61K39/385 ,  A61K39/00 ,  A61K39/0005 ,  A61K39/395 ,  A61K45/06 ,  A61K2039/605 ,  A61K2039/627

摘要： Two-domain MHC polypeptides are useful for modulating activities of antigen-specific T-cells, including for modulating pathogenic potential and effects of antigen-specific T-cells. Exemplary MHC class II-based recombinant T-cell ligands (RTLs) of the invention include covalently linked β1 and α1 domains, and MHC class I-based molecules that comprise covalently linked α1 and α2 domains. These polypeptides may also include covalently linked antigenic determinants, toxic moieties, and/or detectable labels. The disclosed polypeptides can be used to target antigen-specific T-cells, and are useful, among other things, to detect and purify antigen-specific T-cells, to induce or activate T-cells, to modulate T-cell activity, including by regulatory switching of T-cell cytokine and adhesion molecule expression, to treat conditions mediated by antigen-specific T-cells, including treatment and/or prevention of central nervous system damage relating to stroke.

摘要翻译： 双域MHC多肽可用于调节抗原特异性T细胞的活性，包括调节致病潜力和抗原特异性T细胞的作用。 本发明的示例性基于MHC II类的重组T细胞配体（RTL）包括共价连接的1和α1结构域，以及包含共价连接的α1和α2结构域的基于MHC I类的分子。 这些多肽还可以包括共价连接的抗原决定簇，毒性部分和/或可检测的标记。 所公开的多肽可用于靶向抗原特异性T细胞，并且除其他外，可用于检测和纯化抗原特异性T细胞，诱导或激活T细胞以调节T细胞活性，包括 通过T细胞因子和粘附分子表达的调节转换来治疗由抗原特异性T细胞介导的病症，包括治疗和/或预防与中风有关的中枢神经系统损伤。

公开(公告)号：US09492536B2

公开(公告)日：2016-11-15

申请号：US14732145

申请日：2015-06-05

申请人： Oregon Health & Science University ,  The United States of America as represented by the Department of Veterans Affairs

发明人： Halina Offner ,  Patricia D. Hurn ,  Arthur A. Vandenbark ,  Gregory G. Burrows

IPC分类号： A61K39/385 ,  A61K45/06 ,  A61K39/395 ,  A61K38/16 ,  A61K39/00

CPC分类号： A61K39/385 ,  A61K39/00 ,  A61K39/0005 ,  A61K39/395 ,  A61K45/06 ,  A61K2039/605 ,  A61K2039/627

摘要： Two-domain MHC polypeptides are useful for modulating activities of antigen-specific T-cells, including for modulating pathogenic potential and effects of antigen-specific T-cells. Exemplary MHC class II-based recombinant T-cell ligands (RTLs) of the invention include covalently linked β1 and α1 domains, and MHC class I-based molecules that comprise covalently linked α1 and α2 domains. These polypeptides may also include covalently linked antigenic determinants, toxic moieties, and/or detectable labels. The disclosed polypeptides can be used to target antigen-specific T-cells, and are useful, among other things, to detect and purify antigen-specific T-cells, to induce or activate T-cells, to modulate T-cell activity, including by regulatory switching of T-cell cytokine and adhesion molecule expression, to treat conditions mediated by antigen-specific T-cells, including treatment and/or prevention of central nervous system damage relating to stroke.

摘要翻译： 双域MHC多肽可用于调节抗原特异性T细胞的活性，包括调节致病潜力和抗原特异性T细胞的作用。 本发明的示例性基于MHC II类的重组T细胞配体（RTL）包括共价连接的β1和α1结构域，以及包含共价连接的α1和α2结构域的基于MHC I类的分子。 这些多肽还可以包括共价连接的抗原决定簇，毒性部分和/或可检测的标记。 所公开的多肽可用于靶向抗原特异性T细胞，并且除其他外，可用于检测和纯化抗原特异性T细胞，诱导或激活T细胞以调节T细胞活性，包括 通过T细胞因子和粘附分子表达的调节转换来治疗由抗原特异性T细胞介导的病症，包括治疗和/或预防与中风有关的中枢神经系统损伤。