公开(公告)号：US20230071166A1

公开(公告)日：2023-03-09

申请号：US17819539

申请日：2022-08-12

Applicant: The University of Chicago

Inventor： Tong-Chuan He

IPC: C12N15/86 ,  C07K14/245

Abstract: The disclosure is directed to a gene transfer vector which comprises (i) all or part of a viral genome and (ii) a suicide gene flanked by unique cloning sequences. The disclosure also is directed to a system for producing an adenoviral vector comprising a destination vector comprising (i) all or part of an adenoviral genome and (ii) a suicide gene flanked by unique cloning sequences; a transgene flanked by unique cloning sequences; and (c) reagents for Gibson DNA Assembly (GDA). A method of producing an adenoviral vector using the aforementioned system also is provided.