公开(公告)号：US20050101519A1

公开(公告)日：2005-05-12

申请号：US10403003

申请日：2003-04-01

申请人： Therezinha Tomassini ,  Ricardo Dos Santos ,  Milena Soares ,  Deise Cristina Xavier ,  Nancy Barbi ,  Ivone Ribeiro ,  Renata Soares ,  Edmir Fernandez-Ferreira

发明人： Therezinha Tomassini ,  Ricardo Dos Santos ,  Milena Soares ,  Deise Cristina Xavier ,  Nancy Barbi ,  Ivone Ribeiro ,  Renata Soares ,  Edmir Fernandez-Ferreira

IPC分类号： A61K31/00 ,  A61K31/343 ,  A61K31/56 ,  A61K36/81 ,  C07D493/22

CPC分类号： C07D493/22 ,  A61K31/343 ,  A61K31/56 ,  A61K36/81 ,  Y02A50/409 ,  Y02A50/414

摘要： The present invention relates to a process for obtaining Physalis angulata extracts, particularly to isolate ergostane-type steroidal derivatives, such as physalins, which are useful in the treatment of infections caused by protozoans. The physalins of the invention have also prophylactic and therapeutic properties for treating auto-immune diseases in mammals. The isolation process to obtain physalins comprises the steps of: (a) grinding roots, leaves or stem-bark of Physalis ssp; (b) extracting the material obtained in step (a) with solvents selected from the group consisting of water and alcohols, such as methanol, ethanol, 1-propanol, 2-propanol, isobutanol; (c) evaporating the extract obtained in step (b) and washing the syrup material with a suitable solvent; (d) rapid filtering of the extract of step (c) and fractionating the components of the extract according to a polarity gradient of organic solvents; and (e) separating the steroidal components contained in the fractions by chromatography.

摘要翻译： 本发明涉及一种用于获得肌ata提取物的方法，特别是用于分离麦角甾烷型甾体衍生物，例如生物素，其可用于治疗由原生动物引起的感染。 本发明的生理素还具有治疗哺乳动物自身免疫疾病的预防和治疗特性。 获得物质的分离过程包括以下步骤：（a）研磨Physalis ssp的根，叶或茎皮; （b）用选自水和醇的溶剂如甲醇，乙醇，1-丙醇，2-丙醇，异丁醇萃取步骤（a）得到的物质; （c）蒸发步骤（b）中获得的提取物并用合适的溶剂洗涤糖浆料; （d）快速过滤步骤（c）的提取物，并根据有机溶剂的极性梯度对提取物的组分进行分级; 和（e）通过色谱法分离所述级分中所含的甾体组分。