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公开(公告)号：US07655395B2
公开(公告)日：2010-02-02
申请号：US11715725
申请日：2007-03-08
申请人： Timothy Casper , Daniel Cordova , Steven Gutteridge , James J Rauh , Rejane M Smith , Lihong Wu , Yong Tao
发明人： Timothy Casper , Daniel Cordova , Steven Gutteridge , James J Rauh , Rejane M Smith , Lihong Wu , Yong Tao
CPC分类号： C07K14/43563 , C07K14/705
摘要： The genes encoding ryanodine receptor homologs have been characterized from multiple insect families including lepidopteran tobacco budworm (Heliothis virescens), homopteran green peach aphid (Myzus persicae), corn plant hopper (Peregrinus maidis), cotton melon aphid (Aphis gossypil) and fruitfly (Drosophila melanogaster). The full-length genes have been isolated, cloned and amplified in bacterial cells. Expression in insect cells shows that the recombinant protein folds into a functional calcium release channel. The genes and their corresponding polypeptides have a number of uses including, but not limited to, the isolation of other pest ryanodine receptors, the development of screens to identify insecticidally active compounds, use of fragments of genes as pesticides, fragments of protein for antibody production, fragments of protein for determination of the structure of insecticide binding sites, and identification of insecticides that disrupt the calcium balance in cells through other messengers that interact with the receptor calcium release mechanism. Methods are outlined for overcoming toxic effects of expressing recombinant proteins in host cells.
摘要翻译： 编码兰诺定受体同源物的基因已经被多种昆虫家族表征，包括鳞翅目叶烟芽夜蛾（Heliothis virescens），同翅目绿桃蚜（Myzus persicae），玉米植物料斗（Peregrinus maidis），棉瓜蚜虫（Aphis gossypil）和果蝇 黑腹果蝇）。 全长基因已经在细菌细胞中被分离，克隆和扩增。 昆虫细胞中的表达显示重组蛋白折叠成功能性钙释放通道。 基因及其相应的多肽具有许多用途，包括但不限于分离其它害虫兰诺定受体，筛选鉴定杀虫活性化合物，使用基因片段作为农药，用于抗体生产的蛋白质片段 ，用于测定杀虫剂结合位点结构的蛋白质片段，以及鉴定通过与受体钙释放机制相互作用的其他信使破坏细胞中钙平衡的杀虫剂。 概述了克服在宿主细胞中表达重组蛋白的毒性作用的方法。
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公开(公告)号：US20070161037A1
公开(公告)日：2007-07-12
申请号：US11715725
申请日：2007-03-08
申请人： Steven Gutteridge , Timothy Casper , Daniel Cordova , James Rauh , Yong Tao , Lihong Wu , Rejane Smith
发明人： Steven Gutteridge , Timothy Casper , Daniel Cordova , James Rauh , Yong Tao , Lihong Wu , Rejane Smith
CPC分类号： C07K14/43563 , C07K14/705
摘要： The genes encoding ryanodine receptor homologs have been characterized from multiple insect families including lepidopteran tobacco budworm (Heliothis virescens), homopteran green peach aphid (Myzus persicae), corn plant hopper (Peregrinus maidis), cotton melon aphid (Aphis gossypil) and fruitfly (Drosophila melanogaster). The full-length genes have been isolated, cloned and amplified in bacterial cells. Expression in insect cells shows that the recombinant protein folds into a functional calcium release channel. The genes and their corresponding polypeptides have a number of uses including, but not limited to, the isolation of other pest ryanodine receptors, the development of screens to identify insecticidally active compounds, use of fragments of genes as pesticides, fragments of protein for antibody production, fragments of protein for determination of the structure of insecticide binding sites, and identification of insecticides that disrupt the calcium balance in cells through other messengers that interact with the receptor calcium release mechanism. Methods are outlined for overcoming toxic effects of expressing recombinant proteins in host cells.
摘要翻译： 编码兰诺定受体同源物的基因已经被多种昆虫家族表征，包括鳞翅目叶烟芽夜蛾（Heliothis virescens），同翅目绿桃蚜（Myzus persicae），玉米植物料斗（Peregrinus maidis），棉瓜蚜虫（Aphis gossypil）和果蝇 黑腹果蝇）。 全长基因已经在细菌细胞中被分离，克隆和扩增。 昆虫细胞中的表达显示重组蛋白折叠成功能性钙释放通道。 基因及其相应的多肽具有许多用途，包括但不限于分离其它害虫兰诺定受体，筛选鉴定杀虫活性化合物，使用基因片段作为农药，蛋白质片段用于抗体生产 ，用于测定杀虫剂结合位点结构的蛋白质片段，以及鉴定通过与受体钙释放机制相互作用的其他信使破坏细胞中钙平衡的杀虫剂。 概述了克服在宿主细胞中表达重组蛋白的毒性作用的方法。
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