公开(公告)号：US11891602B2

公开(公告)日：2024-02-06

申请号：US17276194

申请日：2019-10-02

Applicant: Toray Industries, Inc.

Inventor： Kyohei Koshimoto ,  Katsuhiko Iseki ,  Hideaki Inada ,  Tatsuya Fujita ,  Keiichi Okimura ,  Munetaka Kunishima ,  Tadaaki Ohgi ,  Eriko Aoki

IPC: C12N15/113

CPC classification number: C12N15/113 ,  C12N2310/531 ,  C12N2330/30

Abstract: A method produces a hairpin single-stranded RNA molecule capable of inhibiting expression of a target gene, including the step of reacting a first single-stranded oligo-RNA molecule represented by formula (I) with a second single-stranded oligo-RNA molecule represented by formula (II) in a mixed solvent including a buffer solution and a hydrophilic organic solvent in the presence of a dehydration condensation agent: 5′-Xc-Lx1 (I) and Lx2-X—Y-Ly-Yc-3′ (II), wherein the dehydration condensation agent is selected from the group consisting of a triazine-based dehydration condensation agent, a uronium-based dehydration condensation agent including an N-hydroxy nitrogen-containing aromatic ring structure, a carbodiimide-based dehydration condensation agent, a 2-halopyridinium-based dehydration condensation agent, and a formamidinium-based dehydration condensation agent.

公开(公告)号：US20240110186A1

公开(公告)日：2024-04-04

申请号：US18459505

申请日：2023-09-01

Applicant: Toray Industries, Inc.

Inventor： Kyohei Koshimoto ,  Katsuhiko Iseki ,  Hideaki Inada ,  Tatsuya Fujita ,  Keiichi Okimura ,  Munetaka Kunishima ,  Tadaaki Ohgi ,  Eriko Aoki

IPC: C12N15/113

CPC classification number: C12N15/113 ,  C12N2310/531 ,  C12N2330/30

Abstract: A method produces a hairpin single-stranded RNA molecule capable of inhibiting expression of a target gene, including the step of reacting a first single-stranded oligo-RNA molecule represented by formula (I) with a second single-stranded oligo-RNA molecule represented by formula (II) in a mixed solvent including a buffer solution and a hydrophilic organic solvent in the presence of a dehydration condensation agent: 5′-Xc-Lx1 . . . (I) and Lx2-X—Y-Ly-Yc-3′ . . . (II), wherein the dehydration condensation agent is selected from the group consisting of a triazine-based dehydration condensation agent, a uronium-based dehydration condensation agent including an N-hydroxy nitrogen-containing aromatic ring structure, a carbodiimide-based dehydration condensation agent, a 2-halopyridinium-based dehydration condensation agent, and a formamidinium-based dehydration condensation agent.

公开(公告)号：US20210317495A1

公开(公告)日：2021-10-14

申请号：US17276194

申请日：2019-10-02

Applicant: Toray Industries, Inc.

Inventor： Kyohei Koshimoto ,  Katsuhiko Iseki ,  Hideaki Inada ,  Tatsuya Fujita ,  Keiichi Okimura ,  Munetaka Kunishima ,  Tadaaki Ohgi ,  Eriko Aoki

IPC: C12P19/34 ,  C12N15/113

Abstract: A method produces a hairpin single-stranded RNA molecule capable of inhibiting expression of a target gene, including the step of reacting a first single-stranded oligo-RNA molecule represented by formula (I) with a second single-stranded oligo-RNA molecule represented by formula (II) in a mixed solvent including a buffer solution and a hydrophilic organic solvent in the presence of a dehydration condensation agent: 5′-Xc-Lx1 (I) and Lx2-X—Y-Ly-Yc-3′ (II), wherein the dehydration condensation agent is selected from the group consisting of a triazine-based dehydration condensation agent, a uronium-based dehydration condensation agent including an N-hydroxy nitrogen-containing aromatic ring structure, a carbodiimide-based dehydration condensation agent, a 2-halopyridinium-based dehydration condensation agent, and a formamidinium-based dehydration condensation agent.