Affinity immobilized metal resins
    2.
    发明授权
    Affinity immobilized metal resins 有权
    亲和固定金属树脂

    公开(公告)号:US06441146B1

    公开(公告)日:2002-08-27

    申请号:US09352620

    申请日:1999-07-13

    Inventor: Tran Quang Minh

    Abstract: Pentadentate chelators (PDC) resins are the metal chelate resins capable of forming the octahedral complexes with several polyvalent metal ions including Cu2+, Ni2+, Zn2+ and Co2+ with five coordination sites occupied by the chelator. This results in the best stability of the complexes and in one coordination site free for interaction and selective binding of accessible cysteine/histidine residues and chiefly histidine containing biomolecules such as proteins or peptides etc. Cu-PDC can be used as concentration resins to reduce the volume of a protein solution. It can be used also as a universal support for immobilizing covalently all proteins, using a soluble carbodiimide.

    Abstract translation: 五齿螯合剂(PDC)树脂是能够与几种多价金属离子(包括Cu 2+,Ni 2+,Zn 2+和Co 2+)形成八面体配合物的金属螯合树脂,其中螯合剂占据五个配位点。 这导致复合物的最佳稳定性,并且在一个配位位点中不存在可接近的半胱氨酸/组氨酸残基和主要含有组氨酸的生物分子如蛋白质或肽等的相互作用和选择性结合。Cu-PDC可用作浓缩树脂以减少 蛋白质溶液的体积。 它也可以用作通过使用可溶性碳二亚胺共价固定所有蛋白质的通用载体。

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