公开(公告)号：US20210002635A1

公开(公告)日：2021-01-07

申请号：US16875418

申请日：2020-05-15

Applicant: Translate Bio, Inc.

Inventor： Jonathan Abysalh ,  Frank DeRosa ,  Jorel Vargas ,  Cameron M. Smith

IPC: C12N15/10

Abstract: The present invention provides, among other things, methods for purifying high quality messenger RNA (mRNA) suitable for clinical use, without using any caustic or flammable solvents. The present invention is, in part, based on surprising discovery that mRNA can be successfully purified by selective precipitation and washing without using ethanol while maintaining integrity and high purity of mRNA. Thus, the present invention provides an effective, reliable, and safer method of purifying RNA from large scale manufacturing process therapeutic applications without using any caustic or inflammable solvents.

公开(公告)号：US20230062449A1

公开(公告)日：2023-03-02

申请号：US17871121

申请日：2022-07-22

Applicant: Translate Bio, Inc.

Inventor： Jonathan Abysalh ,  Frank DeRosa ,  Cameron M. Smith ,  Jorel E. Vargas

IPC: C12N15/10

Abstract: The present invention provides, among other things, methods for purifying high quality messenger RNA (mRNA) suitable for clinical use, without using any caustic or flammable solvents. The present invention is, in part, based on surprising discovery that mRNA can be successfully purified by selective precipitation and washing without using ethanol while maintaining integrity and high purity of mRNA. Thus, the present invention provides an effective, reliable, and safer method of purifying RNA from large scale manufacturing process therapeutic applications without using any caustic or inflammable solvents.

公开(公告)号：US11453877B2

公开(公告)日：2022-09-27

申请号：US16875418

申请日：2020-05-15

Applicant: Translate Bio, Inc.

Inventor： Jonathan Abysalh ,  Frank DeRosa ,  Jorel E. Vargas ,  Cameron M. Smith

IPC: C12N15/10

Abstract: The present invention provides, among other things, methods for purifying high quality messenger RNA (mRNA) suitable for clinical use, without using any caustic or flammable solvents. The present invention is, in part, based on surprising discovery that mRNA can be successfully purified by selective precipitation and washing without using ethanol while maintaining integrity and high purity of mRNA. Thus, the present invention provides an effective, reliable, and safer method of purifying RNA from large scale manufacturing process therapeutic applications without using any caustic or inflammable solvents.

公开(公告)号：US20210324369A1

公开(公告)日：2021-10-21

申请号：US17172527

申请日：2021-02-10

Applicant: Translate Bio, Inc.

Inventor： Jonathan Abysalh ,  Jorel Vargas ,  Cameron M. Smith ,  Joseph Parrella ,  Frank DeRosa

IPC: C12N15/10

Abstract: The present invention provides, among other things, methods for purifying high quality messenger (mRNA) suitable for clinical use. The present invention is, in part, based on surprising discovery that capping and tailing mRNA in reaction buffer having a pH lower than 8.0 and MgCl2 at a concentration of less than 1.25 mM can increase RNA integrity of final mRNA product. Thus, the present invention provides an effective, reliable, and efficient method of manufacturing high quality RNA at large scale for therapeutic use.