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    HOST-ASSOCIATED DNA SEQUENCES, PRIMERS, AND PROBES FOR PCR-BASED IDENTIFICATION OF DOG FECAL POLLUTION SOURCES
    1.
    发明申请
    HOST-ASSOCIATED DNA SEQUENCES, PRIMERS, AND PROBES FOR PCR-BASED IDENTIFICATION OF DOG FECAL POLLUTION SOURCES 有权
    HOST相关DNA序列，引物和探针，用于基于PCR的狗粪污染源鉴定

    公开(公告)号：US20150368695A1

    公开(公告)日：2015-12-24

    申请号：US14231296

    申请日：2014-03-31

    Applicant: U.S. ENVIRONMENTAL PROTECTION AGENCY

    Inventor： Orin C. SHANKS ,  Hyatt C. GREEN

    IPC: C12Q1/68

    CPC classification number: C12Q1/689

    Abstract: A method for detecting dog-fecal contamination in a sample, comprising assaying the sample using a nucleotide sequence based genetic assay which comprises contacting the sample with at least one nucleic acid molecule having the nucleic acid sequence shown in SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, or 11, the nucleic acid sequence being capable of binding to a nucleic acid sequence in the sample, and detecting binding of the nucleic acid molecule to the nucleic acid sequence in the sample, wherein a presence of binding is indicative of the presence of dog-fecal contamination in the sample; the nucleic acid molecules; and a kit comprising at least two of the above-described nucleic acid molecules.

    Abstract translation: 一种用于检测样品中狗粪便污染的方法，包括使用基于核苷酸序列的遗传测定来测定样品，所述方法包括使所述样品与至少一种具有SEQ ID NO：1,2或2所示核酸序列的核酸分子接触， 3个，4个，5个，6个，7个，8个，9个，10个或11个，核酸序列能够结合样品中的核酸序列，并检测核酸分子与核酸序列的结合 样品，其中结合的存在指示样品中粪便粪便的存在; 核酸分子; 以及包含至少两个上述核酸分子的试剂盒。

    HOST-ASSOCIATED DNA SEQUENCES, PRIMERS, AND PROBES FOR PCR-BASED IDENTIFICATION OF DOG FECAL POLLUTION SOURCES
    2.
    发明申请
    HOST-ASSOCIATED DNA SEQUENCES, PRIMERS, AND PROBES FOR PCR-BASED IDENTIFICATION OF DOG FECAL POLLUTION SOURCES 审中-公开

    公开(公告)号：US20180030518A1

    公开(公告)日：2018-02-01

    申请号：US15783595

    申请日：2017-10-13

    Applicant: U.S. Environmental Protection Agency

    Inventor： Orin C. SHANKS ,  Hyatt C. GREEN

    IPC: C12Q1/68

    CPC classification number: C12Q1/689 ,  C12Q2600/158

    Abstract: A method for detecting dog-fecal contamination in a sample, comprising assaying the sample using a nucleotide sequence based genetic assay which comprises contacting the sample with at least one nucleic acid molecule having the nucleic acid sequence shown in SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 0111, the nucleic acid sequence being capable of binding to a nucleic acid sequence in the sample, and detecting binding of the nucleic acid molecule to the nucleic acid sequence in the sample, wherein a presence of binding is indicative of the presence of dog-fecal contamination in the sample; the nucleic acid molecules; and a kit comprising at least two of the above-described nucleic acid molecules.

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