公开(公告)号：US11989959B2

公开(公告)日：2024-05-21

申请号：US17257959

申请日：2019-07-05

申请人： UNIVERSITÄT ZÜRICH

发明人： Lucas Pelkmans ,  Gabriele Gut

IPC分类号： G06V20/69 ,  G01N1/30 ,  G06F18/2137 ,  G06F18/2323 ,  G06V10/762

CPC分类号： G06V20/695 ,  G01N1/30 ,  G06F18/2137 ,  G06F18/2323 ,  G06V10/7635

摘要： The invention relates to a method for processing large multiplexed image data of a biological sample, the method comprising the steps of, recording a plurality of images of a biological sample, wherein the plurality of images comprises images having a different entity of the biological sample targeted with a predefined stain, determining spatially corresponding image pixels in the plurality of registered images, associating the spatially corresponding image pixels to a pixel profile, wherein each pixel profile comprises the pixel values of the spatially corresponding pixels and wherein the pixel profile is associated with the respective image coordinate of the spatially corresponding pixels, pooling the pixel profiles by means of a clustering method configured to determine pixel profiles with similar values, and thereby generating a plurality of clusters, each comprising pixel profiles with similar pixel values, for each cluster assigning a cluster value to the image coordinate of the pixel profiles comprised by said cluster and thereby generating a cluster image with cluster pixels.