公开(公告)号：US20210394434A1

公开(公告)日：2021-12-23

申请号：US17348866

申请日：2021-06-16

Applicant: UNIVERSITY OF CENTRAL FLORIDA RESEARCH FOUNDATION, INC.

Inventor： Swaminathan Rajaraman ,  AVRA KUNDU ,  ADAM ROZMAN ,  JORGE MANRIQUE CASTRO

IPC: B29C64/124 ,  C12M1/32 ,  C12M3/06 ,  C12M1/12 ,  C12M1/42 ,  B33Y10/00 ,  B33Y80/00

Abstract: A method of forming a high-throughput, three-dimensional (3D) microelectrode array for in vitro electrophysiological applications includes 3D printing a well plate having a top face and bottom face. A plurality of culture well each includes a plurality of 3D printed, vertical microchannels and microtroughs communicating with the microchannels. The microtroughs and the microchannels are filled with a conductive paste to form self-isolated microelectrodes in each of the culture wells and conductive traces that communicate with the self-isolated microelectrodes.