公开(公告)号：US10294531B2

公开(公告)日：2019-05-21

申请号：US15027862

申请日：2014-10-07

Applicant: UNIVERSITY OF CENTRAL FLORIDA RESEARCH FOUNDATION, INC.

Inventor： Mollie Jewett ,  Micah Halpern

IPC: G01N1/40 ,  C07K14/20 ,  C12Q1/689 ,  C12Q1/6804 ,  G01N33/569

Abstract: Disclosed herein are method, materials and kits for detection of B. Burgdorferi infection or determining stage of Lyme disease in a subject. Exemplified is a method of diagnosing an infection in a subject, the method involving exposing a biological sample from the subject to a capture substrate under conditions for an infection marker in said biological sample to associate with the capture substrate to form a capture complex; associating said capture complex with a marker complex, said marker complex comprising an oligonucleotide; and amplifying said oligonucleotide of marker complex associated with said capture complex to produce an amplification signal; wherein an amplification signal above a predetermined signal threshold indicates that said subject is infected.