公开(公告)号：US20210338891A1

公开(公告)日：2021-11-04

申请号：US17370101

申请日：2021-07-08

Applicant: UNIVERSITY OF SOUTH CAROLINA

Inventor： DOMINIC M. CASALI ,  MICHAEL A. MATTHEWS

IPC: A61L27/36

Abstract: A system and method for decellularizing tissue is provided. The system includes a pretreatment chamber including a pretreatment solution (e.g., a surfactant), a decellularization solution comprising carbon dioxide and one or more polar solvents, as well as an environmental chamber comprising a treatment chamber. The environmental chamber is maintained at a temperature greater than 31.1° C. and the carbon dioxide is maintained at a pressure greater than 7.38 megapascals to form supercritical CO2. Tissue treated with the decellularization system and method can contain less than 0.05 micrograms of DNA per milligram of dry tissue after the tissue is exposed to the decellularization solution for a time period ranging from about 1 minute to about 2 hours with minimal ECM fiber disruption. A two-part decellularization solution comprising a surfactant as well as supercritical CO2 and one or more polar solvents is also provided.